二球悬铃木花粉变应原特应性体质与HLA-DRB1等位基因相关性分析  

Association between atopy for Platanus Acerifolia pollen and HLA-DRB1 alleles

作　　者：齐名[1] 魏花[2] 朱琴[2] 王艾丽[1] 熊华[1] 

机构地区：[1]南京军区南京总医院中国人民解放军医学检验研究所,南京210002 [2]江苏大学医学技术学院

出　　处：《临床检验杂志》2006年第6期401-403,共3页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(60477031);人事部留学回国人员科研基金资助项目[宁人字2003(37)号]

摘　　要：目的建立对二球悬铃木花粉过敏的特应性体质者的HLA-DRB1等位基因的PCR-SSP检测方法,探讨江苏汉族人二球悬铃木花粉变应原特应性体质者与HLA-DRB1等位基因的相关性。方法用酚-氯仿法提取全血DNA,合成8对等位基因特异性引物,用PCR-SSP方法检测20例江苏汉族二球悬铃木花粉过敏体质者和36例健康人HLA-DRB1*0401,*0402,*0403,*0404,*0405,*0406,*0407,*0408等位基因。结果经优化实验条件,建立了HLA-DRB1的8个等位基因的PCR-SSP检测方法,获得了患者组和健康人组上述HLA-DRB1等位基因的分布资料。二球悬铃木花粉变应原特应性体质者DRB1*0405和*0406基因频率高于健康人对照组而DRB1*0402基因频率低于健康人对照组。其他5个等位基因频率二组间无显著性差异。结论HLA-DRB1*0406和*0405可能是对二球悬铃木花粉变应原过敏的I型变态反应特应性体质者的可疑候选易患等位基因,而DRB1*0402基因可能是与之相关的具有抵抗作用的等位基因。Objective To develop a PCR-SSP method for detection of HLA-DRB1 alleles in the patients who were hypersensitive to Platanus Aeerifolia pollen allergen ,and to probe into the association between the atopic subjects to Platanus Acerifolia pollen allergen and HI,A-DRB1 alleles. Methods DNA in whole blood was extracted by phenol-chlorofor,n method. Eight pairs of specific primers for alleles were synthesized,and HLA-DRB1 ＊ 0401, ＊ 0402, ＊ 0403, ＊ 0404, ＊ 0405, ＊ 0406, ＊ 0407, ＊ 0408 alleles in 20 atopic patients and 36 heahhy individuals of Jiangsu Province with Han nationality were detected by PCR-SSP（ polymerase chain reaction-sequence specific primer）. Results By optimizing the experimental conditions PCR-SSP methods for detection of the 8 alleles were established and the distributing data of above-mentioned HLA DRB1 were obtained. The frequency of HLA DRB1 ＊ 0405 and ＊ 0406 in the patients group was higher than that of in healthy controls group,while the frequency of HLA DRB1 ＊ 0402 in the patients group was lower than that in controis, No significant deference for the other 5 alleles was found between the 2 groups. Conclusion HLA-DRB1 ＊ 0406和 ＊ 0405 seems to he the likely suspected candidate alleles responsible for susceptibility to Platanus Acerifolia pollen allergen in the atopic patients, while DRB1 ＊ 0402 might be contribute to the related resistance to the allergen.

关 键 词：人类白细胞抗原 等位基因 二球悬铃木花粉 变应原 序列特异性引物聚合酶链反应 

分 类 号：R446.6[医药卫生—诊断学]