蕲蛇药材及其市售混淆品的Cyt b基因序列与分析  

Sequence and analysis of Cyt b gene of Agkistrodon and its adulterants sold in market

作　　者：宋文成[1] 宋社吾[2] 刘道芳[2] 刘海兵[1] 宋礼华[2] 

机构地区：[1]安徽大学生命科学学院,安徽合肥230001 [2]安徽省生物研究所,安徽合肥230088

出　　处：《中草药》2006年第12期1862-1865,共4页Chinese Traditional and Herbal Drugs

基　　金：安徽省自然科学基金资助项目(03043101);安徽省优秀青年科技基金资助项目(04043056)

摘　　要：目的 分析薪蛇药材正品及其市场收集样品的Cyt6基因序列，为蕲蛇药材分子鉴别提供依据。方法 利用Cyt6基因，对蕲蛇药材及其市场收集样品进行了序列测定和分析。结果 蕲蛇药材存在着混杂现象；蕲蛇药材正品与其市售混淆品的Cyt6基因序列有着显著差异，该序列在蕲蛇种内差异率为0%～0.91％，与混淆品间的差异率为18．57%～23．78％。结论 Cyt6基因序列是一种鉴别蕲蛇药材与其混淆品较好的分子遗传标记。Objective To differentiate the sequences of Cyt b gene Agkistrodon from its adulterants sold in the market in order to provide molecular evidence for identification of Agkistrodon. Methods Cyt b gene was used to sequence and analyze Agkistrodon and its adulterants sold in the market. Results There was a mixed phenomenon in Agkistrodon species. The differences of Cyt b gene sequence between Agkistrodon and its adulterants sold in the market are significant ： the difference rates among the species of Agkistrodon are 0%-0. 91%, the difference rates between Agkistrodon and its adulterants are 18.57%- 23.78%, Conclusion The characteristics of Cyt b gene sequence can be used as a better molecular marker for authenticating Agkistrodon from its adulterants.

关 键 词：蕲蛇 CYT B基因 分子鉴别 

分 类 号：R282.7[医药卫生—中药学]