大鼠早期心肌缺血CX43免疫组化研究  

Forensic Pathological Significance of Immunohistochemical Study with CX43 In Rats on Early Myocardial Ischaemia

作　　者：金利方[1] 陈明方[2] 郑强[1] 许敬尧[3] 沈永浩[3] 朱有法[3] 

机构地区：[1]杭州市公安局刑事科学技术研究所,浙江杭州310031 [2]杭州市公安局交警支队,浙江杭州310014 [3]浙江大学医学院病理与法医学研究所,浙江杭州310006

出　　处：《法医学杂志》2006年第6期411-412,416,F0003,共4页Journal of Forensic Medicine

摘　　要：目的探讨早期心肌缺血的法医病理学诊断依据。方法通过建立大鼠心肌缺血模型,用免疫组化染色方法检测大鼠心肌缺血15、30、60、120、180min以及正常对照组心肌CX43的表达情况。结果在各组早期心肌缺血区内,见CX43阳性着色的分布与正常组相比,表现为部位的不同及数量上的差异。结论CX43的免疫组化染色对诊断早期心肌缺血有一定的价值,但尚需进一步研究证实。Objective To explore one of evidence for pathologic diagnosis of early myocardial isehaemia. Methods Rats were ligated of the left coronary artery according to a previously documented technique, and heart tissue was sampled at different ischaemia time. The expression of CX43 in myocardial cell was detected by Immunohistochemistry. Results It is showed that the distribution and amount of CX43 positive staining in each group of the myocardial ischaemia was different from that of the control group. Conclusion The changes of CX43 detected by Immunohistochemical methods may be helpful for the diagnosis of early myocardial ischaemia, but further pathologic investigation and research is necessary.

关 键 词：心肌缺血 免疫组化 CX43 

分 类 号：R737.14[医药卫生—肿瘤]