慢性阻塞性肺疾病大鼠模型制备及参七虫草胶囊对肺组织超微结构的影响  

Effect of Shenqichongcao capsule(参七虫草胶囊) on the ultrastructure of pulmonary tissue in rats with chronic obstructive pulmonary disease

作　　者：张念志[1] 周宝银[2] 张一萌[2] 李泽庚[1] 季红燕[1] 李国琳[1] 陈炜[1] 王胜[1] 朱慧志[1] 任薇[1] 彭波[1] 

机构地区：[1]安徽中医学院第一附属医院,安徽合肥230031 [2]安徽中医学院,安徽合肥230038

出　　处：《中国中西医结合急救杂志》2006年第6期323-326,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金资助项目(30472216);安徽省自然科学基金资助项目(03043005)

摘　　要：目的:观察参七虫草胶囊对慢性阻塞性肺疾病(COPD)模型大鼠肺组织超微结构的影响。方法:清洁级W istar大鼠50只被随机分为正常对照组、COPD模型组、参七虫草胶囊高剂量组、参七虫草胶囊低剂量组和金水宝胶囊组,每组10只。采用香烟雾熏加脂多糖(LPS)气管滴入方法复制大鼠COPD模型。利用光镜和透射电镜方法并结合图像分析技术观察各组大鼠肺组织病理形态学变化,气管壁及腺体层厚度,计算线粒体病变率。结果:COPD模型组大鼠肺组织形态出现明显异常改变,线粒体肿胀肥大、空泡变、嵴突病变的发生率明显增高。与COPD模型组相比,参七虫草胶囊高、低剂量组肺组织病理损伤明显减轻;气管壁及腺体层厚度均明显变薄(P<0.01和P<0.05);线粒体肿胀肥大、空泡变、嵴突病变发生率均显著降低(P均<0.05)。金水宝胶囊组肺组织病理损伤较模型组亦有所减轻,但作用不如参七虫草胶囊组显著;线粒体病变发生率与COPD模型组相当。结论:参七虫草胶囊可以延缓和改善COPD模型形成。Objective： To observe the effect of Shenqichongcao capsule （参七虫草胶囊） on the histopathology and ultrastructure of pulmonary tissue in rats with chronic obstructive pulmonary disease （COPD）. Methods： Fifty Wistar rats were randomly divided into normal control group, COPD model group, Shenqiehongeao capsule high dosage group, Shenqiehongcao capsule low dosage group and Jinshuibao capsule （金水宝胶囊） group. Each group had 10 rats. The model of COPD was developed by intra-tracheal instillation of lipopolysaccharide （LPS） twice and exposure to cigarette smoke daily. Optical and transmission electron microscopic methods and image analysis were used to observe pulmonary tissue pathomorphological changes, thickness of tracheal wall and glandular layer and the rate of pathological changes of chondriosome in the rats in the above five groups. Results.. There were obviously abnormal morphological changes in the COPD model group, and the rates of chondriosome enlargement, hypertrophy, vacuole formation and ridge lesion in the pulmonary tissue of the rats in the COPD model group were significantly higher. Compared with the COPD model group, the pulmonary tissue pathological injuries were obviously relieved, the thickness of the tracheal wall and glandular layer was obviously thinner （P〈0.01 and P〈0. 05） and the chondriosome rates of enlargement, hypertrophy, vacuole formation and ridge lesion were conspicuously lowered in the Shenqichongcao high and low dosage groups （all P〈0.05）. Although the pulmonary tissue pathological injuries were also relieved and the rates of pathological changes of chondriosome were comparative in Jinshuibao capsule group as compared with those in the COPD model group, the efficacy of Jinshuibao capsule was not as good as that of Shenqichongcao capsule. Conclusion：Shenqichongcao capsule can delay and improve the formation of COPD model.

关 键 词：肺疾病 阻塞性 慢性 参七虫草胶囊 超微结构 动物模型 

分 类 号：R-332[医药卫生] R285