尿苷酸微生物转化菌株的选育  

Breeding of A Corynebacterium ammoniagenes KU-F14-14 Strain for Microbial Transformation of Uridine Monophosphate

作　　者：钱秀萍[1] 李慧 

机构地区：[1]上海交通大学药学院,上海200240 [2]上海青瑞生物医药技术有限公司,上海200233

出　　处：《中国医药工业杂志》2006年第12期814-816,共3页Chinese Journal of Pharmaceuticals

摘　　要：以产氨短杆菌K13为出发菌株,采用离子注入诱变,并以5-氟胞嘧啶筛选,最终获得了尿苷酸高转化突变株KU-F14-14。突变株生物转化生产尿苷酸能力约为900mg/L,比出发菌株提高了161.6%。A high level of uridine monophosphate （UMP） transformation strain KU-F14-14 was obtained from the original strain Corynebacterium ammoniagenes K13 by mutagenesis with N^＋ ion-beam implantation, followed by selection of resistance to 5-fluorocytosine. The results showed that the productivity of UMP by the transformation of mutant KU-F14-14 was about 900mg/L, which had increased by 161.6% over the original strain.

关 键 词：尿苷酸 产氨短杆菌 离子注入 诱变育种 

分 类 号：TQ464.4[化学工程—制药化工]