Survivin及Bcl-2基因在非小细胞肺癌中的表达和相关性  

Expression and correlativity of Survivin and Bcl-2 in human non small cell lung cancer

作　　者：刘静[1] 王艳[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院,150040

出　　处：《现代生物医学进展》2006年第11期131-134,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨Survivin基因的表达在肺癌发生、发展中的作用及其Bcl-2蛋白表达的相互关系。方法:应用PT-PCR检测Sur- vivin mRNA的表达。结果:表达阴性的肺癌病人总生存率明显高于阳性表达的病人,并且其表达与患者年龄、性别、吸烟史、组织类型、肿瘤分化、大小及纵隔淋巴结转移无显著相关。免疫组化显示,肿瘤细胞胞浆和胞核中Survivin阳性表达分别为70％和80％,胞浆和胞核同时阳性为54％,Survivin在胞浆中的免疫活性与肿瘤分期有关(P=0．019),与患者生存期无关。在所有类型的肺癌中,鳞状细胞癌中的Survivin明显增高。用免疫组织化学链霉茵抗生物素蛋白过氧化酶法(SP法)检测Bcl-2蛋白的表达。Bcl-2在鳞癌中的阳性表达率为最高。结论:Survivin基因在非小细胞肺癌中表达上调,提示其通过抑制细胞凋亡,在肺癌癌变发生、发展中起重要作用,可能成为肺癌基因治疗的新靶点,其阳性表达亦预示肿瘤有较高的侵袭性和不良预后,Survivin基因与凋亡抑制基因Bcl2的共同表达可能在肺癌中起协同作用。Objective： To investigate the expression of Survivin and its relationship with expression of B-cell lymphoma-2 （Bcl-2） in human non-small-cell lung carcinoma （NSCLC）. Methods： The expression of the Survivin mR.NA was detected by RT- PCR technique. Results： Survival ratios of the patients of lung cancer with negative expression were significandy higer than those with positive expression ,which is not significantly related to ages, gender, smoking history, histology, lymphatic metastasis, Immunohistochemistry indicated that the positive expression of Survivin in cytoplasm and in nucleus in NSCLC was 70% and 80% respectively; the simultaneous positive expression of the two was 54%, The immunoreactivity of Survivin in the cytoplasm was related to TNM stages but not related to survival time, The expression of Survivin in squamous cell carcinoma （SCC） was significandy higher than other types of SCC, The positive expression rate of Bcl-2 in squamous cell carcinoma was the highest, Conclusion： Survivin Gene may play an important role in the pathway of carcinogenesis and progression of NSCLC and it may become a new therapeutic target in genic treatment of pulmonary cancer.The up-regulation of the expressinon of Survivin predicts more invasion and poor prognosis. Sirvivin and Bcl-2 might play synergetic roles in NSCLC.

关 键 词：肺肿瘤 免疫组织化学 SURVIVIN基因 BCL-2基因 

分 类 号：R734.2[医药卫生—肿瘤]