微生物酶法生产L-半胱氨酸的产酶条件优化  

Optimization of Fermentation Conditions of Microbial Enzymatic Synthesis of L-cysteine

作　　者：寇广会[1] 怀丽华[1] 白钢[2] 杨文博[2] 

机构地区：[1]天津市工业微生物重点实验室天津科技大学生物工程学院,天津300457 [2]南开大学生命科学学院,天津300071

出　　处：《天津科技大学学报》2006年第4期21-24,共4页Journal of Tianjin University of Science & Technology

基　　金：天津市科委应用基础研究重点基金资助项目(05YFJZJC00901)

摘　　要：以假单胞菌(Pseudom onas sp.)TS1138为供试菌株,对微生物酶法生产L-半胱氨酸的条件进行了初步研究。通过对产酶培养基中的碳氮源进行研究,得到了TS1138菌株产酶的最佳碳氮源分别为葡萄糖和尿素,DL-ATC的最适添加量为5 g/L;通过对产酶培养基的产酶条件进行研究,得出了最适的种子接种量为10%,产酶培养基的最适初始pH为8.0,500 mL三角瓶的最适装液量为40 mL。Pseudomonas sp. TS1138 was used as the test strain to produce L-cysteine, and microbial enzymatic synthesis of L-cysteine was studied. In the medium optimization, it was found that glucose and urea were the best carbon and nitrogen source for enzyme production, respectively. Effect of DL-ATC on enzyme production was carried out, and the experimental result showed that a initial concentration of 5 g/L DL-ATC was found to be optimal for enzyme production. Effect of inoculum size, initial pH and liquid volume on enzyme production were investigated. The results indicated that the optimum inoculum size was 10%,and the optimum initial pH was 8.0 ,and the optimum liquid volume was 40 mL culture medium in a 500 mL shaking flask.

关 键 词：假单胞菌 酶法合成 L-半胱氨酸 

分 类 号：Q93[生物学—微生物学]