微孔板杂交技术检测乙肝病毒X基因及其临床应用价值  

HBV X Gene and Its Clinical Application Value Detected with Millipore Plate Hybridization

作　　者：宋玉国[1] 张吉林[1] 黄建文[1] 毕胜利[1] 

机构地区：[1]北华大学附属医院,吉林吉林132011

出　　处：《北华大学学报（自然科学版）》2006年第6期535-537,共3页Journal of Beihua University（Natural Science）

基　　金：吉林省科技厅资助项目(19990316-03)

摘　　要：目的建立微孔板杂交技术检测乙肝病毒X基因的方法，研究其临床应用价值．方法在微孔板上包被HBVX基因片段的捕获探针；PCR扩增被检标本中目的片段，其引物用Bio-Ⅱ-dUTP修饰，在微孔板上杂交后用链霉亲和素碱性磷酸酶系统显色．结果该方法检测HBVX基因灵敏，特异；乙肝后肝细胞癌患者X基因阳性率明显高于急、慢性乙肝和肝纤维化组．结论检测HBVX基因对HCC具有辅助诊断价值．Objective HBV X gene and its clinical application value were detected with millipor plate hybridization. Methods Capture probe was coated HBV X gene fragment on millipor plate; PCR amplified objective fragment of examined sample. Bio-Ⅱ-dUTP modified its primer. After hybridization on millipor plate, streptavidin ALP system colored. Results The method detected HBV X gene sensitiveness; X gene of positive rate of HCC patients is obviously more than acute and chronic HBV and hepatic fibrosis. Conclusion Detecting HBV X gene possess auxiliary diagnosis for HCC.

关 键 词：乙肝病毒 X基因 肝细胞癌 

分 类 号：R512[医药卫生—内科学]