XPD基因多态性与非吸烟女性肺癌易感性的关系  

Association of genetic polymorphism in the DNA repair gene XPD with risk of lung cancer in nonsmoking females

作　　者：尹智华[1] 马锐[2] 崔泽实[3] 李鸣川[1] 何钦成[1] 周宝森[1] 

机构地区：[1]中国医科大学流行病教研室,沈阳110001 [2]辽宁省肿瘤医院 [3]中国医科大学实验技术中心

出　　处：《中国肺癌杂志》2006年第6期492-496,共5页Chinese Journal of Lung Cancer

基　　金：美国中华医学基金会(No.00-726);国家自然科学基金(No.30471493)资助~~

摘　　要：背景与目的 着色性干皮病互补基因D（xeroderma pigmentosum group D，XPD）是一种重要的DNA损伤修复基因，其常见的多态是位于751密码子的A→C多态。本研究旨在探讨XPD基因751位点单核苷酸多态性与非吸烟女性肺癌易感性的关系，并探讨油烟暴露与基因多态性交互作用对肺癌风险的影响。方法 采用病例-对照研究方法，纳入非吸烟女性肺癌患者105人和对照105人。以聚合酶链反应-限制性片段长度多态性方法分析XPD基因Lys751Gln多态基因型。结果 携带至少1个751Gln等位基因者患肺癌的风险显著增高，调整OR为2．80（95％CI为1．21～6．48）。携带等位基因751Gln又有油烟暴露的个体患肺癌的风险较两个危险因素单独作用时更高，校正OR为6．85（95％CI为1．69～27．67，P=0．007）。结论 XPD基因Lys751Gln多态是非吸烟女性肺癌的遗传易感因素。携带XPD751Gln等位基因又有油烟暴露的非吸烟女性患肺癌的风险明显增高。Background and objective Xeroderma pigmentosum group D （XPD） is one of the important DNA repair genes. XPD polymorphism at Lys751Gln site has been shown to alter XPD protein function, modulate DNA repair capacity and therefore affect cancer risk. The aim of this study is to explore the relationship between XPD polymorphism and susceptibility to lung cancer in nonsmoking female via a population-based case-control study. Methods There were 105 female patients who were diagnosed with lung cancer between January 2004 and December 2005 from Liaoning Tumor Hospital and 202 Hospital, and the control group included 105 healthy volunteers who were obtained from community centers at the same time. Information concerning demographic and risk factors was obtained for each case and control by a trained interviewer. XPD genotypes of cases and controls were determined by PCR RFLP method. Two-sided Z2 test was used to compare the distribution of the genotypes and risk factors between cases and controls. Unconditional logistic regression analysis was performed to calculate the odds ratios （OR） with 95% confidence intervals （CI） for estimating the association between certain genotypes and lung cancer and exploring the interaction of environmental risk factors and genetic polymorphism. Results All of the subjects in this study were nonsmoking females in Shenyang. There was no significant demographic difference （age, economic level and education） between cases and controls. There was a significant difference in the frequencies of XPD polymorphism between cancer cases and controls. The frequencies of XPD 751Gln allele were 6.2% in controls and 13.8% in cases （P〈0.05）. The risk of lung cancer was higher in those with the Lys/Gln or Gln/Gln genotype than in those with the Lys/Lys genotype and adjusted OR was 2.80 （95% CI： 1.21-6.48）. The result showed that cooking fumes exposure was a risk factor for lung cancer （OR was 2.44）. Furthermore, an interaction between environmental risk factors and the variant

关 键 词：女性肺癌 XPD基因 遗传多态性 

分 类 号：R734.2[医药卫生—肿瘤]