大鼠急性心肌梗死心室重塑中细胞因子与胶原的变化  

Changes of cytokine and collagen in the myocardial remodeling after acute myocardial infarction in rats

作　　者：郭张强[1] 廖玉华[1] 程翔[1] 李彬[1] 葛洪霞[1] 刘英[1] 张玲[1] 王敏[1] 郭和平[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心内科,湖北武汉430022

出　　处：《中国病理生理杂志》2006年第12期2322-2327,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30370574)

摘　　要：目的:探讨大鼠急性心肌梗死(M I)后心室重塑过程细胞因子与胶原分子的关系。方法:制备雄性W istar大鼠心肌梗死和假手术对照模型,于手术后3 d、1 w、4 w分别观察不同时点梗死区和非梗死区的Ⅰ、Ⅲ型胶原及炎症因子的mRNA变化。结果:心肌梗死后第3 d胶原(Ⅰ、Ⅲ型胶原)和细胞因子(TGF-β1、TNF-α)皆上升,梗死区Ⅰ、Ⅲ型胶原在第4周仍然比非梗死区为高,TGF-β1、TNF-α在第1周达到高峰后逐渐下降但仍高于同期假手术组(P<0.01),相关性分析提示梗死区和非梗死区TGF-β1、TNF-α均与Ⅰ、Ⅲ型胶原存在相关(P<0.01)。结论:炎症因子参与心室重塑的病理生理过程,干预细胞因子可能作为早期防治心室重塑的手段。AIM： To clarify the relationship between the cytokine and collagen in myocardial remodeling after acute myocardial infarction （MI） in rats. METHODS： In MI group, Wistar rats were undergone acute myocardial infarction by ligation of the anterior descending coronary artery. Sham operation was made in rats as control. The mRNA expression of collagen and cytokines such as TNF -α and TGF -β1 in infract and non - infarct region of left myocardium were detected by RT -PCR at different time point （3 d, 1 and4 weeks）. RESULTS： Collagen type Ⅰ and Ⅲ elevated as well as the TNF -α and TGF - β1 in the MI group at 3th day. Expression of collagen type Ⅰ and Ⅲwere higher in the infarct region than that in the non - infarct region even at 4 weeks. TNF -α and TGF - β1 peaked at 1 week and declined gradually to the baseline, which was still higher than those in control group （P 〈0. 01 ）. Correlation analysis revealed that expressions of TNF -α and TGF - β1 were positively correlated with the collagen type Ⅰ and 19 （ P 〈 0. 01 ）. CONCLUSION ： Cytokines participate in the myocardial remodeling after MI. Interfering with expression of cytokines may be the potentially preventative method in the myocardial remodeling.

关 键 词：心肌梗死 心室复建 胶原 细胞因子类 

分 类 号：R363[医药卫生—病理学]