脾切除对门脉高压症大鼠CD4、CD8和脾功能的影响  

Effects of splenectomy on CD4^+,CD8^+ and spleen function in cirrhotic rats with portal hypertension

作　　者：王文擘[1] 郑树森[1] 贾长库[1] 丁光超[2] 

机构地区：[1]浙江大学医学院附属第一医院卫生部多器官联合移植研究重点实验室,浙江杭州310003 [2]浙江大学附属儿童医院,浙江杭州310031

出　　处：《中国病理生理杂志》2006年第12期2360-2363,共4页Chinese Journal of Pathophysiology

基　　金：浙江省科学技术厅院士基金资助项目(No.2003A1008-07)

摘　　要：目的:研究肝硬化门脉高压症(PHT)大鼠脾次全切除术后CD4、CD8与脾功能的变化。方法:皮下注射60%四氯化碳复制大鼠肝硬化模型。共分5组:正常大鼠组、肝硬化(PHT)组、正常大鼠脾切除组、PHT全脾切除组、PHT脾次全切除组,每组10只。手术后第4周检测各组血常规、肝功能、tuftsin、CD4、CD8等指标。结果:PHT高压症大鼠脾切除术后tuftsin水平[(171±21)ng/Lvs(433±44)ng/L,P<0.01]、CD4/CD8(2.01±0.22vs1.12±0.12,P<0.01)均显著低于PHT组;PHT大鼠脾次全切除组tuftsin[(434±42)ng/Lvs(171±21)ng/L,P<0.01]、CD4/CD8(1.97±0.18vs1.12±0.12,P<0.01)显著高于PHT大鼠脾切除组。肝功能两者无显著差异(P>0.05)。结论:PHT脾次全切组大鼠术后免疫和脾功能比PHT脾全切组大鼠明显改善,肝功能两者无显著变化。AIM： To investigate the effect of subtotal spleneetomy on the expression of CD4 ^＋ ,CD8 ^＋ and tuftsin in eirrhosie rats with portal hypertension （PHT） . METHODS： Rats liver cirrhosis was induced by subcutaneous injection of 40% CCl4. Fifty rats were randomly divided into five groups （n = 10）. Group A： control rats; group B： PHT rats; group C： normal rats with total spleneetomy; group D： PHT with total spleneetomy and group E： PHT with subtotal spleneetimy. The hepatic function, the expression of CD4 ^＋ , CD8 ^＋ , the ratio of CD4^＋ to CD8 ^＋ and tuftsin were analyzed at the fourth week after treatment. RESULTS： The expression of tuftsin , the ratio of CD4 ^＋ to CD8 ^＋ was significantly decreased in PHT rats with total splenectomy compared with PHT rats [ （ 171 ± 21 ） ng/L vs （433 ± 44 ）ng/L,P 〈 0. 01 ; （2. 01 ± 0. 22 vs 1.12 ±0. 12） ,P 〈0. 01 ]. In the group of PHT rats with subtotal spleneetomy, the expression of tuftsin, the ratio of CD4^＋ to CD8^＋ was higher than those in the PHT rats with total splenectomy [ （434 ±42） ng/L vs （ 171 ± 21 ） ng/L,P 〈0.01 ; （ 1.97 ±0. 18 vs 1.12 ±0. 12,P 〈0. 01 ], however, the hepatic function was not show difference（ P 〉 0. 05 ）. CONCLUSION ： Spleen and immune function is significantly improved in PHT rats after subtotal splenectomy, but the hepatic function is not changed significantly.

关 键 词：肝硬化 高压症 门静脉 脾切除术 免疫功能 

分 类 号：R363[医药卫生—病理学]