急性白血病患者同源结构域相互作用蛋白激酶2的表达及其临床意义  

HIPK2 gene expression and its clinical significance in acute leukemia

作　　者：邢海燕[1] 张新伟[1] 田征[1] 唐克晶[1] 秘营昌[1] 王敏[1] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所血液病医院实验血液学国家重点实验室,天津300020

出　　处：《中国病理生理杂志》2006年第12期2364-2367,共4页Chinese Journal of Pathophysiology

基　　金：天津市自然基金资助项目(No.043607311)

摘　　要：目的:探讨急性白血病患者中同源结构域相互作用蛋白激酶2(H IPK2)基因的表达及其临床意义。方法:应用半定量逆转录-聚合酶链反应(RT-PCR)检测急性白血病患者(AL)及健康供者骨髓单个核细胞(MNC)H IPK2基因的表达。结果:①初发AL患者H IPK2的相对转录水平为0.364±0.286,显著低于正常对照组(1.160±0.272)(P<0.01);②AML患者中M2b的H IPK2的转录水平显著高于其它类型的AML(P<0.05);③除M2b外的AL患者H IPK2的转录水平与FAB亚型、发病时外周血白细胞数、细胞或分子遗传学异常、乳酸脱氢酶水平、多药耐药基因1(mdr1)表达、P170表达及年龄等预后因素无显著相关性(P>0.05)。结论:H IPK2在AL患者中低表达,可能与AL的发生发展有关。AIM： To investigate homeodomain - interacting protein kinase 2 （ HIPK2 ） gene expression level and its clinical significance in acute leukemia （AL） patients. METHODS： HIPK2 mRNA was determined by semi - quantitative reverse transcriptase polymerase chain reaction （semi -quantitive RT- PCR） in patients with acute leukemia and healthy donors. The relative transcription level was compared between the study group and the control group. RESULTS： ① The relative expression level of HIPK2 mRNA in AL patients was 0. 364 ± 0. 286, significantly lower than that in control （1. 160 ±0. 272, P 〈0. 01 ）. ② HIPK2 gene expression level in M2b type was significantly higher than that in other acute myeloid leukemia （AML） subtypes （P 〈 0. 05）.③ HIPK2 gene expression in AL, except M2b, had no relationship with the clinical prognostic factors such as cytogenetic or molecular aberrations, WBC counts, LDH, mdrl, P170 protein and age （ P 〉 0. 05 ）. CONCLUSION ： AL has significantly lower HIPK2 mRNA expression as compared to normal control, which may be associated with leukemogenesis and/or disease progression of AL.

关 键 词：白血病 基因 HIPK2 基因表达 

分 类 号：R363[医药卫生—病理学]