去氢表雄酮及G6PD基因反义寡核苷酸对Raji细胞的某些影响  

Effects of DHEA and G6PD antisense oligodeoxynucleotides on Raji cells

作　　者：张德太[1] 胡丽华[1] 杨渝珍[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院,湖北武汉430022 [2]华中科技大学同济医学院生物化学与分子生物学系,湖北武汉430030

出　　处：《中国病理生理杂志》2006年第12期2397-2400,共4页Chinese Journal of Pathophysiology

基　　金：湖北省自然科学基金资助项目(No.2004ABA189)

摘　　要：目的:观察去氢表雄酮(DHEA)及G6PD基因的全硫代修饰反义寡核苷酸对体外培养Raji细胞的抑制作用。方法:分别用不同浓度DHEA及G6PD反义寡核苷酸作用于体外培养Raji细胞,观察细胞存活、生长情况,同时采用逆转录-聚合酶链反应检测Raji细胞G6PD基因mRNA表达水平,测定G6PD活性。结果:DHEA及G6PD反义寡核苷酸不影响Raji细胞存活,50μmol/L、500μmol/L浓度的DHEA作用72 h小时及10.0μmol/L反义寡核苷酸作用48 h后均明显抑制Raji细胞生长(P<0.01);当DHEA≥5.0μmol/L时,作用Raji细胞72 h,则G6PD活性明显低于未用DHEA作用的对照组(P<0.01),但不影响G6PD基因mRNA表达;10.0μmol/L反义寡核苷酸作用Raji细胞48 h后,G6PD基因mRNA表达水平低于未用反义寡核苷酸作用的对照组,G6PD活性亦明显低于对照组(P<0.01)。结论:一定浓度的DHEA及G6PD反义寡核苷酸均能抑制Raji细胞G6PD活性并不同程度影响细胞生长,但其抑制机制不同。AIM： To investigate the suppressive effects of dehydroepiandrosterone （DHEA） and glucose -6 - phosphate dehydrogenase （G6PD） antisense oligodeoxynucleotides on Raji cells. METHODS： Raji cell line was cultured in vitro in the presence of DHEA at different concentrations ranged from 0. 05 μmol/L to 500 μmol/L or G6PD antisense oligodeoxynucleotides. The viability and proliferation of the cells pretreated with dehydroepiandrosterone or G6PD antisense oligodeoxynucleotides were evaluated. Meanwhile, intracellular activities and mRNA expression of G6PD were analyzed. RESULTS ： DHEA and G6PD antisense oligodeoxynucleotides does not influence the viability of cells in culture. Raji cells treated with DHEA at concentration of 50 μmol/L or 500 μmol/L for 72 h or with 10.0 μmol/L G6PD antisense oligodeoxynucleotides for 48 h had significant lower cell numbers compared with control （ P 〈 0. 01 ）. Raji cells treated with DHEA at concentration more than 5.0 μmol/L for 72 h had significant decreased G6PD activities （ P 〈 0. 01 ） but no change in mRNA expression levels was observed. With 10. 0 μmol/L G6PD antisense oligodeoxynucleotides pretreatment for 48 h, the G6PD mRNA expression levels and activities were significantly decreased （ P 〈 0. 01 ）. CONCLUSION ： DHEA or G6PD antisense oligodeoxynucleotides at specific concentration have suppressive effects on G6PD activities and proliferation in Raji cells to a certain extent, but the suppressive mechanisms are different.

关 键 词：基因 寡核苷酸类 反义 RAJI细胞 葡萄糖磷酸脱氢酶 普拉睾酮 

分 类 号：R363[医药卫生—病理学]