人工牛黄对实验性惊厥大鼠脑内海马及门区神经元丢失的抑制和GAD免疫反应阳性细胞的保护作用研究  

Artificial Calculus Bovis inhibits neuron loss in hippocampus and hilus and protects the GAD positive cells in hippocampus of epileptic rats

作　　者：梁洁芳[1] 胡景鑫[1] 杨彬源[1] 陈盛强[2] 钟愉[1] 梁玉鹃[1] 邓广斐[3] 钟蓓华[3] 黄哲[3] 黄兰英[3] 郭蓝蓝[3] 

机构地区：[1]广州医学院生理教研室,广东广州510182 [2]广州医学院第二附属医院神经研究所,广东广州510260 [3]广州医学院药理教研室,广东广州510182

出　　处：《中国病理生理杂志》2006年第12期2414-2417,共4页Chinese Journal of Pathophysiology

摘　　要：目的:研究人工牛黄对致痫大鼠行为的影响,海马及门区神经元丢失情况和谷氨酸脱羧酶(GAD)免疫反应阳性细胞数目的改变,探讨该药的抗惊厥作用。方法:采用戊四唑急性惊厥模型,参照Rac ine标准观察行为学表现,用N issl染色作海马及门区神经元计数,用免疫组织化学方法作GAD免疫反应阳性细胞计数。结果:牛黄防治组惊厥发作的潜伏时间较对照组长,发作次数较惊厥组少,而在海马及门区的神经元和在海马的GAD阳性细胞的丢失数量较惊厥组的少。结论:人工牛黄能延长惊厥发作的潜伏期,减少发作次数,减轻神经元丢失和保护GAD免疫反应阳性细胞。AIM ： To probe into the anti - epilepsy action of artificial Calculus Bovis, by observing its effect on the behavioral of the experimental epileptic rats, neuron loss in the hippocampus and hilus, and GAD positive cell alteration in the hippocampus. METHODS ： SD rats were divided into three groups ： group A （ artificial Calculus Bovis treatment group） ; group B （acute epilepsy group） and group C （control group）. A model of acute epilepsy rats was established by PTZ. The rat＇s behavioral alteration was observed by the Racine＇ scale. The neurons in the hippocampus and hilus were calculated by Nissl staining. The GAD positive cells were observed by immunohistochemical staining. RESULTS： The latency of the first seizure in group A was longer than that in group B, while the seizure times in group A was less than that in group B. Besides, in group A, both the neuron loss amount in the hippocampus and hilus and the GAD positive cell loss amount in the hippocampus were less than those in group B. CONCLUSION： The artificial Calculus Bovis prolonged the latency of the first seizure time, decreased the frequency of seizure, and prevented the neuron loss and protected the GAD positive cells.

关 键 词：人工牛黄 惊厥 谷氨酸脱羧酶 免疫组织化学 

分 类 号：R363[医药卫生—病理学]