丙酮酸乙酯对脓毒症小鼠肝细胞的保护作用  

Hepatocyte protection of ethyl pyruvate in septic mice

作　　者：陈敏英[1] 周奇[2] 梁力建[3] 彭宝岗[3] 管向东[1] 欧阳彬[1] 陈娟[1] 

机构地区：[1]中山大学附属第一医院外科重症监护中心,广东广州510080 [2]中山大学附属第一医院黄埔院区外一科,广东广州510700 [3]中山大学附属第一医院肝胆外科,广东广州510080

出　　处：《中国病理生理杂志》2006年第12期2450-2452,共3页Chinese Journal of Pathophysiology

摘　　要：目的:研究丙酮酸乙酯(ethyl pyruvate,EP)对脓毒症小鼠肝细胞的保护作用。方法:制作小鼠盲肠结扎穿孔模型,应用丙酮酸乙酯林格氏液(REPS)与乳酸钠林格氏液(RLS)对小鼠进行液体复苏,检测肝组织的抗氧化能力和肝功能改变。结果:脓毒症小鼠抗氧化能力低于假手术组,P<0.01。应用REPS复苏显著提高肝组织抗氧化能力,REPS组肝组织丙二醛浓度低于RLS组[(48.18±5.98)μmol.g-1prote invs(78.34±11.16)μmol.g-1pro-te in],超氧化物歧化酶[(5.19±1.41)103U/g prote invs(3.20±1.08)103U/g prote in]和总抗氧化能力[(7.02±1.79)103U/g prote invs(4.77±1.35)103U/g prote in]高于RLS组,P<0.01;REPS组小鼠丙氨酸转氨酶低于RLS组,[(210.06±23.36)Uvs(458.86±51.55)U],P<0.01。结论:丙酮酸乙酯具有显著的抗氧化效应,提高脓毒症小鼠肝细胞的抗氧化能力,改善肝功能。AIM： To study the protective effect of ethyl pyruvate （EP） on hepatocytes in septic mice. METHODS： The cecal ligation - perforation was made in mice as septic model. Ringer＇s ethyl pyruvate solution （REPS） and Ringer＇s lactic solution （RLS） were used to resuscitate septic mice. Anti - oxidative capacity of hepatic tissue and liver function were detected in different groups. RESULTS ： Anti - oxidative capacity in septic mice was significantly lower than that in sham group （P 〈0. 01 ）. EP promoted the anti - oxidative capacity of hepatic tissue in septic mice. Malondialdehyde level was lower in REPS group than that in RLS group [ （48.18 ±5.98） μmol ·g^-1 protein vs （78.34 ±11. 16） μmol ·g^-1 Protein ] , superoxide dismutase [ （5. 19 ± 1.41 ） 10^3 U/g protein vs （ 3.20 ± 1.08 ） 10^3 U/g protein ] and total anti - oxidative capacity [ （7.02 ± 1.79） 10^3 U/g protein vs （4. 77 ± 1.35 ） 10^3 U/g protein] level were higher in REPS group than those in RLS group （ P 〈 0. 01 ）. Alanine aminotransferase in REPS group were lower than that in RLS group [ （ 210.06 ± 23.36） U vs （458. 86 ±51.55） U, P 〈0. 01]. CONCLUSION： Ethyl pyruvate is an effective anti- oxidant in septic mice, which significantly increases the anti -oxidative capacity in hepatic tissue and ameliorates liver function.

关 键 词：脓毒症 肝细胞 丙酮酸乙酯 

分 类 号：R363[医药卫生—病理学]