大鼠垂体前叶内神经纤维对LH分泌的兴奋性调节作用  

Excitatory regulative effect of nerve fibers on LH secretion in rat anterior pituitary

作　　者：刘玲[1] 高立志[1] 鞠躬[1] 

机构地区：[1]第四军医大学全军神经科学研究所,陕西西安71003

出　　处：《第四军医大学学报》2006年第23期2143-2145,共3页Journal of the Fourth Military Medical University

基　　金：陕西省自然科学基金(2000FM25)

摘　　要：目的:探讨垂体前叶神经纤维对黄体生成素(LH)分泌的神经调节.方法:阴道涂片筛选出动情间期大鼠80只,随机等分为假手术组(SO)和去卵巢组(OVX),术后7d处死,取出垂体前叶,用Krebs液或含河豚毒素(TTX)的Krebs液离体灌流,同时施加10Hz或2Hz,时间为10min或2min的电场刺激(EFS).从施加EFS前10min开始,每10min收集灌流液1次,连续收集130min,放免法检测LH浓度.结果:Krebs液单纯灌流时,SO组的LH平均浓度为(4.8±0.5)IU/L,OVX组LH平均浓度为(7.1±1.3)IU/L,两组间存在显著差异(P<0.05).10Hz持续10min的EFS后10min内两组的LH浓度较基础水平有显著增高.SO组由(4.9±0.1)IU/L升高至(15.7±0.8)IU/L;OVX组由(7.5±4.7)IU/L升高至(20.0±1.4)IU/L(P<0.05).而改用含TTX的Krebs液灌流后,同参数的EFS后10min内LH浓度在SO组和OVX组分别为(5.4±0.5)IU/L和(7.7±0.9)IU/L,提示其分泌被显著抑制.其余各参数的EFS对LH分泌均无明显影响.结论:垂体前叶内神经纤维可能对LH的分泌存在直接的调节.AIM： To investigate the direct regulative effect of nerve fibers on luteinizing hormone （LH） secretion in rat anterior pituitary. METHODS： Eighty diestrus rats determined by vaginal smear were randomly divided into sham operation （SO） and ovariectomy （OVX） groups. Seven days after operations, the anterior pituitary was harvested and superfused with Krebs solution （Krebs-Ringer-bicabonate-BSA buffer, KRBGA） with or without tetrodotoxin （Trx）. Electrical field stimulation （EFS） was performed at 10 Hz or 2 Hz for 10 min or 2 min, respectively, and the perfusate was continuously collected every 10 min for 130 min from the time 10 min before EFS and measured for LH by radioimmunoassay. RESULTS： In the anterior pituitary superfused with KRBGA without EFS, LH concentration were in a steady basal level with difference between the 2 groups, （4.8 ±0.5） IU/L in SO group, and （7.1 ±1.3） IU/L in OVX group （P 〈0.05）. EFS at 10 Hz for 10 min significantly increased LH secretion in the t/me 10 min after cease of stimulation, from （4.9 ±0.1 ） IU/ L to （ 15.7 ±0.8） IU/L in SO group and from （7.5 ±4.7） IU/L to （20.0 ± 1.4） IU/L in OVX group, respectively （ P 〈 0.05 ）. However, the effect of the same parameter of EFS was abolished if the Trx was added into the perfusate, and LH concentration decreased to （5.4 ±0.5） IU/L in SO group and （7.7 ±0.9） IU/L in OVX group 10 min after cease of stimulation. Other variables of EFS have no effect on LH secretion. CONCLUSION： The nerve fibers in the anterior pituitary may have a direct regulative effect on LH secretion.

关 键 词：电场刺激 黄体生成素 垂体前叶 神经纤维 卵巢 切除术 河豚毒素 大鼠 

分 类 号：Q575.12[生物学—生物化学]