右旋甲硫氨酸与紫杉醇联合应用治疗胃癌  

The effect of combination of D-methionine and docetaxel on gastric cancer cells

作　　者：刘冀红[1] 曹伟新[1] 纪玉宝[2] 刘炳亚[2] 朱正纲[3] 林言箴[3] 

机构地区：[1]上海第二医科大学附属瑞金医院临床营养科,上海200025 [2]上海第二医科大学附属瑞金医院上海消化外科研究所,上海200025 [3]上海第二医科大学附属瑞金医院普通外科,上海200025

出　　处：《肠外与肠内营养》2006年第6期338-341,共4页Parenteral & Enteral Nutrition

摘　　要：目的:研究右旋甲硫氨酸(D-Met)与紫杉醇联用治疗胃癌的效果。方法:选用D-Met培养液培养7株胃癌细胞5d,用流式细胞仪检测细胞周期;然后再用D-Met培养液培养7株胃癌细胞达96h后,添加紫杉醇继续培养24h,用氮四唑(MTT)法检测其吸光度(A490)。另设L-Met培养液组为阴性对照组,Met-培养液组为阳性对照组,不添加紫杉醇组为平行对照组。结果:与L-Met组相比,D-Met培养液组中除NCI-N87细胞的S期比例显著增高(P<0.01)外,其余6株胃癌细胞的G2/M期比例均显著增高(P<0.05或P<0.01);Met-培养液组的7株胃癌细胞的G2/M期比例均显著增高(P<0.05或P<0.01)。添加紫杉醇的D-Met和Met-培养液中,各株胃癌细胞抑制率均显著高于同种培养液不加药组(P<0.05)和添加紫杉醇的L-Met培养液组(P<0.01);D-Met培养液加紫杉醇组对MKN45细胞的抑制率显著高于Met-培养液加紫杉醇组(P<0.05)。结论:D-Met环境作用于胃癌细胞5d,可将大多数胃癌细胞阻滞于G2/M期,其效果与Met-环境相似;D-Met与紫杉醇联用可起到协同作用,对于MKN45细胞D-Met与紫杉醇联用的效果大于Met-与紫杉醇联用。Objective：To investigate the effect of combination of D-methionine and docetaxel on gastric cancer cells. Methods：First, seven gastric cancer cell lines were cultured with three different kinds of media （ D-Met, L-Met and Met） for five days, and cell cycle was detected by flow cytometry. Second, gastric cancer cells were respectively cultured in L-Met, D-Met, and Metmedia for 96 h, then docetaxel was added into 3 media. After 24 h, proliferation was measured by MTT method. Results：All gastric cancer cell lines were arrested in G2/M phase （P 〈0.05 ,P 〈0.01 ） after cultured in D-Met medium for 5 days except NCI-N87 in S phase （P 〈0.01 ）. All gastric cancer cell lines in Met medium were arrested in G2/M phase （P 〈0.05,P 〈 0.01 ） . Along with docetaxel, the inhibitory rates of gastric cancer cells in D-Met or Met medium were significantly higher than those in the same medium without docetaxel （ P 〈 0.05） and those in L-Met medium with docetaxel （P 〈 0.01 ）. The inhibitory effect of combination use of D-Met and docetaxel for MKN45 was superior to the combination of Met and docetaxel. Conclusion：Most gastric cancer cells were arrested in G2/M phase after five days＇culture with D-Met medium, which was similar to that in Met medium. It was more efficient using D-Met along with docetaxel to inhibit the proliferation of gastric cancer cells in treatment. This combination use of D-Met was superior to the combination Met for MKN45 cell line.

关 键 词：右旋甲硫氨酸 胃癌 紫杉醇 

分 类 号：R735.2[医药卫生—肿瘤]