中性粒细胞趋化因子在急性胰腺炎相关急性肺损害中的作用  

Role of cytokine-induced neutrophil chemoattractant in acute lung injury during acute pancreatitis

作　　者：徐岷[1] 周国雄[2] 张尤历[1] 张建新[3] 何亚龙[1] 黄介飞[2] 张弘[2] 魏群[2] 

机构地区：[1]江苏大学附属医院消化内科,江苏省镇江市212001 [2]南通大学附属医院消化内科,江苏省南通市226001 [3]江苏大学附属医院普外科,江苏省镇江市212001

出　　处：《世界华人消化杂志》2006年第34期3273-3277,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨中性粒细胞趋化因子(CINC)在急性胰腺炎相关的急性肺损害(ALI)中的作用.方法:分别采用ip雨蛙肽和胰胆管逆行注射50g/L牛磺胆酸钠建立大鼠轻症、重症急性胰腺炎模型.84只SD大鼠随机分为雨蛙肽组、生理盐水组、牛磺胆酸钠组和手术对照组.分别检测各组不同时间点血清淀粉酶、肺干湿重比和肺组织病理学改变,用免疫组织化学法和半定量逆转录聚合酶链反应(RT-PCR)法检测肺组织中CINC蛋白和CINCmRNA表达的变化情况.结果:雨蛙肽组各时间点CINC蛋白和CINCmRNA的表达与生理盐水组无明显差异(P>0.05);牛磺胆酸钠组与手术对照组相比,血清淀粉酶、肺干湿重比显著升高(P<0.05),术后1h肺组织CINCmRNA的表达开始升高(1h:0.23±0.07vs0.07±0.04,P<0.05;3h:0.36±0.07vs0.06±0.04,P<0.05;6h:0.56±0.07vs0.09±0.05,P<0.01;12h:0.49±0.09vs0.11±0.03,P<0.01),术后3h开始有CINC蛋白的表达,随着时间的延长表达逐渐增强,且与肺组织的病理改变呈正相关.结论:CINC可能在胰腺炎相关的急性肺损害中起了重要的作用.AIM： To investigate the role of cytokine-induced neutrophil chemoattractant （CINC） in the pathogenesis of acute lung injury （ALI） during acute pancreafitis （AP）. METHODS： AP model was induced by intraperitoneal injection of cerulein or retrograde infusion of 50 g/L sodium taurocholate into the bili-pancreatic duct in Sprague Dawley rats, A total of 84 rats were randomly divided into 4 groups： cerulein group, saline control group, sodium taurocholate group and operational control group. The level of serum amylase, the pulmonary dry/wet weight ratio and histological changes were measured at different time points in each group. The expression of pulmonary CINC protein and mRNA were detected by immunohistochemistry and semiquantitative reverse transcriptase polymerase chain reaction （RT-PCR）, respectively. RESULTS： No significant difference was found between cerulein group and saline control group at different time points （P 〉 0.05）. In comparison with those in operational control group, the level of serum amylase and pulmonary dry/wet weight ratio were significantly higher in sodium taurocholate group I h after operation （P 〈 0.01）, and the expression of pulmonary CINC mRNA started to increase at the Ist h after operation （1 h： 0.23±0.07 vs 0.07 ± 0.04, P 〈 0.05; 3 h：0.36 ± 0.07 vs 0.06 ± 0.04, P 〈 0.05; 6 h： 0.56 ± 0.07 vs 0.09 ± 0.05, P 〈 0.01; 12 h： 0.49 ± 0.09 vs 0.11 ± 0.03, P 〈 0.01）. The expression of pulmonary CINC protein was detected at 3^rd h after operation, and then up-regulated gradually. Moreover, the expression of CINC was correlated with pulmonary histological changes. CONCLUSION： CINC plays an important role in the pathogenesis of ALI during AP.

关 键 词：急性胰腺炎 急性肺损害 中性粒细胞趋化因子 免疫组化 半定量逆转录聚合酶链反应 

分 类 号：R576[医药卫生—消化系统]