FK506对人视网膜微血管内皮细胞增殖及凋亡的影响  

The Effect of FK506 on the Proliferation of HRCECs

作　　者：马红婕[1] 李涛[1] 梁小玲[1] 丁小燕[1] 谢素贞[1] 唐仕波[1] 

机构地区：[1]中山大学中山眼科中心,510060

出　　处：《眼科学报》2006年第4期237-243,共7页Eye Science

基　　金：国家自然科学基金(30471849);中国博士后科学基金(2005037614);教育部重点专项科研基金[教技发中心函(2003)172号]资助

摘　　要：目的:观察FK506对常规及高糖培养条件下的人视网膜微血管内皮细胞(HRCECs)的增殖状态、细胞周期及凋亡的影响。方法:分离培养HRCECs,并应用第Ⅷ因子相关抗原抗体鉴定。取P3-4代HRCECs进行高糖培养(30mmol/l右旋葡萄糖)培养,应用MTT法检测其生长状态。应用100pM的FK506对常规培养的高糖培养条件的HRCECs进行干预,通过MTT法、流式细胞仪检测其对生长状态、细胞周期及凋亡的影响。结果:FK506可抑制常规及高糖培养条件下HRCECs的增殖,其可抑制高糖培养下细胞周期,但可同时减少凋亡的发生,但对常规培养的细胞周期及凋亡则无影响。结论:上述结果提示FK506可能有抑制新生血管发生的作用,成为增殖性糖尿病视网膜病变的有效药物。Purpose： To observe the effect of FK506 on the proliferation, cell cycle and apoptosis of HRCECs under the cultural conditions of routine and hyperglucose. Methods： Primary cultures of HRCECs were prepared from donor human eye for corneal transplantation and identified by immunohistochemistry Ⅷ factor fos-related antigenantibody. The viability of HRCECs under the cultural conditions of hyperglucose was analyzed by MTT assay. The effects of FK506 on the reproductive activity of HRCECs under different cultural conditions were studied by MTT assay. And the effects of FKS06 on the cell cycle and apoptosis were analyzed by flow cytometry with DNA stained by propidiun iodide （PI） and Annexin V-FITC. Results： The reproductive activity of HRCECs was inhibited under cultural condition of hyperglucose. The reproductive activity of HRCECs was significantly inhibited by 100 pM FK506 under the cultural conditions of routine or hyperglucose. The cell cycle of HRCECs under cultural condition of hyperglucose can be suppressed by FK506, as well as the apoptosis can be inhibited, but not under the routine condition. Conclusion： The results of the research suggest FK506 can be a hopeful drug for PDR treatment. Eye Science 2006;22：237-243.

关 键 词：糖尿病 血管内皮细胞 FK506 细胞周期 

分 类 号：R774.1[医药卫生—眼科]