二甲胺四环素对嗅感觉神经元凋亡的保护作用  

Protective effects of minocycline against apoptosis of olfactory sensory neurons

作　　者：叶放蕾[1] 郭向东[1] 娄卫华[1] 谢天飞[1] 

机构地区：[1]郑州大学第一附属医院耳鼻咽喉科,郑州450052

出　　处：《临床耳鼻咽喉科杂志》2006年第24期1135-1137,共3页Journal of Clinical Otorhinolaryngology

摘　　要：目的:观察二甲胺四环素对慢性鼻窦炎大鼠嗅感觉神经元(OSNs)凋亡的保护作用。方法:将大鼠随机分为对照组(A组)、鼻窦炎造模组(B组)和鼻窦炎加二甲胺四环素组(C组),于不同时间取材,用苏木精-伊红染色观察嗅上皮厚度及上颌窦黏膜病理变化,用免疫组织化学染色检测Caspase-3表达阳性细胞。结果:苏木精-伊红染色镜检发现上颌窦黏膜病理学改变:A组明显轻于B组和C组,B、C两组嗅上皮厚度差异有统计学意义(P<0.05),免疫组织化学镜检发现B、C两组Caspase-3阳性细胞表达差异有统计学意义(P<0.05)。结论:二甲胺四环素能抑制鼻窦炎大鼠模型Caspase-3的表达,可能成为治疗嗅觉障碍的有效药物。Objective：To investigate the protective effects of minoeycline against apoptosis of olfactory sensory neurons induced by sinusitis. Method： Sixty adult SD rats were divided randomly into three groups： control group （group A）, sinusitis group （group B） and intravenous injection of minocycline group （group （2）. The rats were sacrificed at 1.3.5 d and 7 d after surgery, and then the tissue sections of olfactory epithelium were stained and evaluated with hematoxylin and eosin （HE） and immunohistoehemistry method. Result：The histopathological changes in maxillary sinus epilhelium tissues observed with he staining microscopic examination in groupse Band C were worse than that in group A. Significant difference of the thickness of olfactory epitheliual and Caspase-3 activity was demonstrated in group B and group C. Conclusion：The result demonstrated that minocycline could inhibit expression of Caspase-3 in rats with sinusitis.

关 键 词：鼻窦炎 二甲胺四环素 嗅感觉神经元 细胞凋亡 CASPASE -3 

分 类 号：R765.6[医药卫生—耳鼻咽喉科]