定量检测T细胞受体重排删除DNA环含量评价急性非淋巴细胞白血病患者胸腺近期输出功能  

Evaluation of thymic recent output function in patients with ANLL by quantitative analysis of TRECs content

作　　者：杨力建[1] 李扬秋[1] 陈少华[1] 耿素霞[1] 吴秀丽[1] 张学利[1] 

机构地区：[1]暨南大学医学院血液病研究所,广州510632

出　　处：《现代临床医学生物工程学杂志》2006年第4期342-345,共4页Journal of Modern Clinical Medical Bioengineering

基　　金：国家自然科学基金(30270579);广东省自然科学基金重点项目(23001);广东省教育厅"千百十工程"优秀人才培养基金项目(Q校02022)

摘　　要：目的检测急性非淋巴细胞白血病(ANLL)患者T细胞受体重排删除DNA环(signaljoint T-cell receptor exc ision DNA c irc les sjTRECs,TRECs)的含量,从而探讨患者的初始型naive T细胞水平和胸腺近期输出功能特点。方法利用实时定量PCR(TaqM an)方法,检测77例ANLL患者外周血单个核细胞(PBMC)TRECs的水平,并根据外周血中CD3阳性率计算CD3细胞中TRECs水平。14例ANLL缓解期患者和14例正常人外周血作为对照。结果ANLL患者外周血中TRECs含量为(0.43±0.92)拷贝/1 000 PBMCs,(2.02±3.25)拷贝/1 000 CD3+细胞,明显低于正常人TRECs水平[(4.10±3.65)拷贝/1 000 PBMCs和(6.84±4.71)拷贝/1 000 CD3+细胞,P=0.0000和P=0.0001]和缓解期患者[(2.19±2.49)拷贝/1 000 PBMCs和(6.30±7.13)拷贝/1 000 CD3+细胞,P=0.0000和P=0.0005]。各亚型ANLL患者的TREC水平差异无统计学意义(P>0.05)。结论绝大多数ANLL型患者胸腺近期输出naive T细胞功能明显降低,缓解期患者的胸腺近期输出功能有一定程度恢复。Objective To investigate the level of T-cell receptor excision DNA circles （TRECs） in patients with acute non-lymphocytic leukemia （ ANLL）, thereby to evaluate the content of naive T cells and the recent thymic output function. Methods Quantitative detection of TRECs in DNA of peripheral blood mononuclear cells （ PBMC ） from 77 cases with ANLL was preformed by real-time PCR （ TaqMan ） analysis. And the TRECs number was related to the number of T-cells by determination of the number of CD3-positive cells. Fourteen cases with completive remission of ANLL （ANLL-CR） and 14 normal individuals served as controls. Results Compared with normal individuals [ （4.10 ± 3.65 ） copies/1 000 PBMCs and （6.84 ± 4. 71 ） copies/1 000 CD3 ^＋ cells, P = 0. 0000 and P = 0. 0001 ] and ANLL-CR patients [ （ 2. 19 ± 2.49） copies/1 000 PBMCs and （6.30± 7.13 ） copies/1 000 CD3^＋ cells,P = 0. 0000 和 P=0. 0005 ] , a dramatic reduction of TRECs values in patients with ANLL [ （0.43 ± 0. 92） copies/1 000 PBMCs and （2.02 ± 3.25 ） copies/1 000 CD3 ^＋ cells ] could be found. There was no significant difference of the TRECs level among different subtypes of ANLL （ P 〉 0. 05 ）. Conclusion Thymic recent output naive T cells function reduces prominently in ANLL and recovers in some degree during CR phase.

关 键 词：T细胞受体重排删除DNA环 胸腺近期输出功能 白血病 实时定量PCR 

分 类 号：R733.71[医药卫生—肿瘤]