CP8304亚型人乳头瘤病毒感染与子宫颈病变的关系  

Relationship of HPV CP8304 and cervical lesions

作　　者：魏灵荣[1] 马佳[2] 王平[1] 

机构地区：[1]北京市海淀医院检验中心 [2]北京市海淀医院宫颈病变中心,北京100080

出　　处：《临床和实验医学杂志》2006年第12期1917-1918,共2页Journal of Clinical and Experimental Medicine

摘　　要：目的通过基因分型检测,研究CP8304亚型人乳头瘤病毒(HPV)的感染特点及其与子宫颈病变的关系。方法对细胞学检查结果异常的患者,取其宫颈脱落细胞,经聚合酶链反应(PCR)扩增后与21种HPV亚型探针进行反斑点印迹杂交,进行基因分型检测,从中选取CP8304阳性的病例57例,对分型结果进行分析,并与细胞学结果比较。结果57例CP8304亚型阳性病例中,15例(26.3%)为独立感染,42例(73.7%)为混合感染。HPV16和58是最常见的混合感染亚型。入选病例的细胞学检查结果为:3例(5.3%)炎症,28(49.1%)非典型磷状细胞(ASCUS),22例(38.6%)低度鳞状上皮细胞内病变(LSIL),4例(7.0%)高度病变(HSIL)。结论CP8304亚型HPV感染常引起较轻的细胞学变化,但由于具有易与其它亚型混合感染的特性,也不排除发展为高度病变的可能。Objective Infective characteristics of CP8304 and its relationship with cervical lesions were evaluated by genotyping. Methods Exfoliated cells of cervix uteri were collected from women who had cytological abnormalities. HPV genotypes were analyzed by reverse dot blot with 21 HPV genotype probes of the PCR product. Fifty - seven CP8304 genotype positive samples were analyzed and compared with cytological results, Results Among 57 CP8304 positive cases, 15 （26.3%） were single genotype, 42 （73.7%） were multi - genotype. HPV 16 and HPV 58 coinfection were most frequent. Compared with cytological results, 3 （5.3%） cases were inflammation, 28 （49.1%） were ASCUS, 22 （38.6%） were LSIL, 4 （7.0%） were HSIL. Conclusion HPV CP8304 infection usually results in low - grade cervical lesions. However, there are also possibilities of developing into high - grade lesions because of its co - infected characteristic.

关 键 词：人乳头瘤病毒 基因分型 HPV—GP8304 宫颈病变 

分 类 号：R737.33[医药卫生—肿瘤]