新构建的含DF3启动子和白喉毒素A片段的重组表达载体对人乳腺癌细胞的特异性杀伤作用  

SPECIFIC KILLING EFFECT OF RECOMBINANT EXPRESSION VECTOR CONTAINING DF3 PROMOTOR AND DIPHTHERIA TOXIN A FRAGMENT ON HUMAN BREAST CANCER CELLS

作　　者：蔡明[1] 黄文广[1] 罗威[1] 潘晟[1] 殷涛[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院普外科,武汉430022

出　　处：《中国组织化学与细胞化学杂志》2006年第6期638-643,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：湖北省卫生厅科研基金资助项目(NX200501)

摘　　要：目的研究新构建的含人乳腺癌DF3启动子和白喉毒素A片段的重组表达载体PGL3-DF3-DTA对人乳腺癌细胞的影响。方法构建重组表达载体PGL3-DF3-DTA,并用其转染DF3阳性和阴性的乳腺癌细胞MCF-7和MDA-MB-231。通过MTT法测定PGL3-DF3-DTA在体外对乳腺癌细胞生长的影响,建立裸鼠动物模型观察PGL3-DF3-DTA在体内对乳腺癌细胞的杀伤效应。结果成功构建出重组表达载体PGL3-DF3-DTA并建立了人乳腺癌裸鼠动物模型,经重组表达载体PGL3-DF3-DTA作用后的DF3阳性人乳腺癌细胞出现明显的凋亡现象,Ki-67、bcl-2基因表达水平降低,bax基因表达水平升高。结论重组表达载体PGL3-DF3-DTA能对DF3阳性的乳腺癌细胞产生特异性杀伤作用。Objective To study the effects of the recombinant expression vector containing human breast cancer DF3 promotor and diphtheria toxin A fragment on human breast cancer cells. Methods The recombinant expression vector PGL3-DF3-DTA was constructed and transfected into DF3 positive and negative human breast cancer cells. MTT colorimetry was used to examine the effect of PGL3-DF3-DTA on the growth of human breast cancer cells. The nude mouse model was established to observe the killing effect of PGL3-DF3-DTA on human breast cancer cells. Results The recombinant expression vector PGL3-DF3-DTA was constructed successfully, and human breast cancer xenografts were established in nude mice. The expression of Ki-67 and bcl-2 were downregulated while the expression of bax was elevated in the DF3-positive human breast cancer cell line. Conclusion The recombinant expression vector PGL3-DF3- DTA can produce a specific killing effect on the DF3-positive human breast cancer cell line.

关 键 词：DF3 白喉毒素A片段 基因表达 基因治疗 人乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]