甜菜碱对干旱胁迫下湖北海棠超微弱发光及抗氧化能力的影响  

Effects of glycinebetaine on ultraweak luminescence and anti-oxidative capability of Malus hupehensis under drought stress.

作　　者：接玉玲[1] 赵海洲[1] 张伟[1] 杨洪强[1] 李德全[1] 束怀瑞[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018

出　　处：《应用生态学报》2006年第12期2394-2398,共5页Chinese Journal of Applied Ecology

基　　金：山东省优秀中青年科学家奖励基金项目(02BS021);国家自然科学基金资助项目(30270923;30571285).

摘　　要：以盆栽4年生的苹果砧木湖北海棠(Malushupehensis)为试材,叶面喷施100mmol·L-1的甜菜碱,研究外源甜菜碱对干旱胁迫下湖北海棠叶片超微弱发光(UWL)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢(H2O2)、脯氨酸(Pro)及甜菜碱(GB)含量的影响.结果表明,轻度干旱胁迫下,叶片UWL强度提高,但随着干旱胁迫程度的加重而下降,至严重干旱胁迫时UWL明显低于正常供水处理;H2O2、MDA含量随干旱胁迫加重而升高;SOD和POD活性随胁迫加重而升高,但至重度干旱时下降.与单独干旱处理相比,叶片喷施甜菜碱显著提高了轻度和中度干旱胁迫叶片的UWL(分别提高35·27%和43·95%)、SOD和POD活性,降低了H2O2和MDA含量;促进了Pro和GB的积累.表明甜菜碱及通过甜菜碱诱导的脯氨酸积累,能够提高干旱胁迫下叶片的抗氧化能力,减轻过氧化损伤,对叶片细胞起到保护作用.In a pot experiment with 4-year-old apple root stock （Malus hupehensis） , this paper studied the effects of foliarspraying exogenous glycinebetaine （GB） on the leaf ultraweak luminescence （UWL） , contents of malondialdehyde （ MDA）, hydrogen peroxide （ H2O2 ） , endogenous GB and proline （Pro） , and activities of superoxide dismutase （SOD） and peroxidase （POD） under drought stress. The results showed that UWL intensity increased under slight- and mid drought stress, but decreased with increasing drought stress till lower than the control. Under slight- and mid drought stress, foliar-spraying GB improved the UW＇L intensity by 35.27% and 43.95% , respectively, decreased H2O2 and MDA contents, increased SOD and POD activities, and promoted the accumulation of GB and Pro. The exogenous GB and its induced Pro accumulation could increase the anti-oxidative capability of M. hupehensis leaves, and protect the leaf cells from oxidative damage under drought stress.

关 键 词：干旱胁迫 超微弱发光 甜菜碱 抗氧化能力 湖北海棠 

分 类 号：S661.1[农业科学—果树学]