bax基因对人BcaCD885颊癌细胞凋亡的影响  

Apoptosis of BcaCD885 Cell Mediated by Human bax Gene

作　　者：边莉[1] 金克炜[2] 何永文[3] 

机构地区：[1]昆明医学院第一附属医院病理科,650032 [2]昆明医学院病理学教研室 [3]昆明医学院口腔医学研究所

出　　处：《肿瘤防治研究》2006年第12期880-882,共3页Cancer Research on Prevention and Treatment

基　　金：云南省教育厅科学研究基金资助项目(0112444)

摘　　要：目的探讨bax基因对人BcaCD885颊癌细胞凋亡的影响。方法转染人bax-α基因重组质粒及对照质粒于BcaCD885细胞中。通过HE染色光镜观察对细胞形态学进行检测;通过TUNEL原位末端标记及流式细胞术对细胞凋亡进行定性、定量检测。结果bax质粒转染组BcaCD885细胞出现凋亡的形态学改变,TUNEL阳性红染凋亡细胞数量增多,凋亡小体出现,其细胞凋亡率与空白对照组及对照质粒转染组之间存在极显著差异(P<0.01)。结论bax-α基因重组质粒能促进BcaCD885细胞发生凋亡。Objective To study the change of apoptosis by bax gene in human buccal mucosa carcinoma BcaCD885 cells. Methods Transfected recombinant human Bax gene plasmid and contral plasmid into BcaCD885 cells. The BcaCD885 cells were examined morphologically through haematoxylin-eosinophil （HE） staining. The apoptosis qualification of the BcaCD885 cells was examined through terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling technique （TUNEL）. The apoptosis rate of the BcaCD885 cells was measured with flow cytometry. Results The bax gene transfected BcaCD885 cells had apoptosis morphological features. Many TUNEL positive red staining cells were found in the bax gene transfected BcaCD885 cells. There were significant differences in the apoptotic rates between the bax gene transfected BcaCD885 and the control BcaCD885 cells and the control plasmid transfected BcaCD885 cells （P〈0. 01）. Conclusion The recombinant human bax gene plasmid pORF-h bax-α can promote the apoptotic rate of the BcaCD885 cells.

关 键 词：BAX基因 BCACD885细胞 细胞凋亡 

分 类 号：R73-362[医药卫生—肿瘤] R739.81[医药卫生—临床医学]