Simvastatin抑制白介素-6的产生促进大鼠坐骨神经再生  

Simvastatin effect on inhibiting of the production of serum IL-6 and promotion the regeneration of sciatic nerve of rats

作　　者：赵智[1] 赵慧[2] 吕淑红[2] 秦绍春[1] 张健[2] 丛庆伟[2] 李爱萍[2] 

机构地区：[1]大连医科大学第二临床学院骨科,辽宁大连116027 [2]大连医科大学生理学教研室脑疾病研究所,辽宁大连116027

出　　处：《中国矫形外科杂志》2006年第24期1891-1893,1905,共4页Orthopedic Journal of China

基　　金：大连市科技计划资助项目(No.2003B3NS222)

摘　　要：[目的]探讨他汀类(statins)药物S imvastatin促进大鼠坐骨神经修复及其免疫调节机制。[方法]制作SD大鼠坐骨神经钳夹损伤(crush)模型,分别予S imvastatin和溶媒(0.3%羧甲基纤维素钠)对照干预2周,并设立假手术组。手术后作行为学、神经电生理学、组织学计价和血清TNFα-和IL-6检测。[结果]S imvastatin干预组趾展功能指数在术后5、8 d较对照组大,2周肌肉复合动作电位(CMAP)幅度高,4周神经传导速度(NCV)快;手术后5 d,血清IL-6和TNFα-水平均低于对照组,尤以IL-6为明显;S imvastatin干预组神经再生形态优于对照组。[结论]S imvastain可能通过减少血清IL-6和TNFα-的生成,抑制免疫反应,对大鼠坐骨神经crush损伤修复产生促进作用。[ Objective] To study the effect of Simvastatin on the regeneration of sciatic nerve of rats and its mechanism of immunoregulation. [ Method ] Twenty eight female Sprague-Dawley rats were used and the rats experienced the sciatic nerve crush injury. Animals were randomized into： Simvastatin-treated crush injury animals, vehicle-treated crush injury animals and shamoperated animals. Simvastatin （20 mg/kg） was given once a day over a time period of 14 day sby oral garage via a pharyngeal tube. After surgery, the functional evaluation of nerve recovery, electrophysiologic assessment, histological assessment, serum IL- 6 and TNF-α assessment were performed. [ Result] The toe spread index of Simvastatin-treated crush injury animals was higher significantly than that of vehicle rats at d5 and d8 （ P 〈 0.05 ） postoperatively. CMAP was higher and NCV was faster （ P 〈 0. 05 ） ; The serum IL-6 and TNF-α of at 5d of post-operation was significant lower, especially IL-6. The pathological changes showed the numbers of myelinated axons of Simvastatin-treated crush injury animals were more than that of vehicle animals. [ Conclusion]The present study showed that Simvastatin may promote the regeneration of the sciatic nerve after crush injury in rats partly through lowering the levels of serum IL-6 and TNF-α.

关 键 词：SIMVASTATIN 坐骨神经crush损伤 肌肉复合动作电位 IL-6 TNF—α 

分 类 号：R96[医药卫生—药理学]