N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸对大鼠心脏成纤维细胞胶原蛋白合成及表达的调节作用  

Mediating effect of AcSDKP on collagen synthesis in cultured rat cardiac fibroblasts

作　　者：王丽平[1] 朱曦龄[1] 杨方[1] 王瑞敏[1] 马文东[1] 罗玲[1] 户万秘[1] 李治国[1] 李琪佳[1] 裴新[1] 张丽娟[1] 王献华[1] 

机构地区：[1]华北煤炭医学院实验研究中心,唐山063000

出　　处：《解剖学杂志》2006年第6期718-720,740,共4页Chinese Journal of Anatomy

基　　金：河北省科技厅博士基金(04547002D-5);河北省教育厅基金(2003112);唐山市新医药基础研究重点实验室项目(04362001B)

摘　　要：目的：探讨N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(AcSDKP)对血小板衍生生长因子(PDGF)诱导的大鼠心脏成纤维细胞胶原合成的调节作用。方法：建立新生大鼠心脏成纤维细胞系；采用~3H-脯氨酸掺入法检测心脏成纤维细胞胶原蛋白的合成。采用Western印迹法检测心脏成纤维细胞Ⅰ、Ⅲ型胶原蛋白的表达。结果：PDGF对心脏成纤维细胞胶原蛋白合成的促进作用与浓度相关,并以10 ng/ml时最强。AcSDKP对PDGF介导的心脏成纤维细胞胶原合成有抑制作用,并且在10^(-9)mol/L时作用最强。结论：AcSDKP对PDGF介导的心脏成纤维细胞胶原合成有明显抑制作用,可能与其抗心脏纤维化的作用相关。Objective： To investigate whether AcSDKP can inhibit collagen synthesis in cultured rat cardiac fibroblasts mediated by platelet derived growth factor （PDGF）. Methods： Neonatal rat cardiac fibroblasts were isola ted. The synthesis of collagen was measured by ^3H proline incorporation assay. The expression of type Ⅰ and Ⅲ collagen protein was detected by Western blot. Results： PDGF stimulated cardiac fibroblasts collagen synthesis in a dose-dependent manner, in which 10 ng/ml PDGF reached its peak. AcSDKP inhibited cardiac fibroblasts collagen synthesis mediated by PDGF. 10-9 mol/L AcSDKP attained its peak when inhibiting cardiac fibrohlasts colla gen synthesis in cardiac fibroblasts. Conclusion： AcSDKP can inhibit collagen synthesis in cultured rat cardiac fibroblasts mediated by PDGF.

关 键 词：N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸 血小板衍生生长因子 心脏成纤维细胞 胶原蛋白 

分 类 号：R363[医药卫生—病理学]