bcl-2、mtp53在Ⅰ型神经纤维瘤病中的表达及意义  

The expression and significance of Bcl-2 and P53 in typeⅠ neurofibromatosis

作　　者：朱格非[1] 王玉荣[2] 麦慧[1] 黄欣[1] 梁敏华[1] 

机构地区：[1]广西壮族自治区人民医院整形外科,南宁530021 [2]武汉华中科技大学同济医学院附属协和医院整形外科

出　　处：《广西医学》2006年第12期1876-1878,共3页Guangxi Medical Journal

摘　　要：目的了解bcl-2、突变型p53(mtp53)在Ⅰ型神经纤维瘤病(NF-Ⅰ)中的表达及意义,探讨它们在NF-Ⅰ中的作用。方法对9例孤立性神经纤维瘤、10例NF-Ⅰ及10例正常皮肤进行免疫组化(SP法)染色,观察bcl-2、mtp53在不同组织中的表达。结果bcl-2、mtp53在正常皮肤中均表达阴性。bcl-2在NF-Ⅰ中表达(100%)与孤立性神经纤维瘤(55·6%)之间的差异有统计学意义(P<0·01)。mpt53在NF-Ⅰ(70·0%)和孤立性神经纤维瘤(44·4%)之间的阳性表达差异无统计学意义,两者与正常皮肤之间的差异有统计学意义(P<0·01)。结论bcl-2、mtp53在NF-Ⅰ的发生及发展中起重要作用。推测bcl-2可能与细胞核膜上相应受体结合,并在其它多种细胞因子的作用下抑制瘤细胞的凋亡,造成肿瘤细胞生存延长,细胞数目增多而使瘤体持续的增大和复发。而在NF-Ⅰ中野生型p53(wild-type p53,wtp53)可能已突变成mtp53,从而转化细胞,抑制凋亡,加速肿瘤细胞生长。Objective To investigate the expression of bcl-2 and mtp53 in NF- Ⅰ , and to make further understanding of their role in pathogenesis of neurofibromatosis. Methods Expression of bcl-2 and mtp53 was analyzed with lesions samples from patients with the solitary neurofibroma（9 case） and NF-Ⅰ （10 case）, compared with ten normal skin samples, using immunohistochemieal method （SP technique）. Results There were no positive expression of bcl-2 and mtp53 in normal skin samples. The expression of bcl-2 were higher in NF-Ⅰ （100%） than in solitary neumfibmma（55.6% ）. The expression of in solitary neurofibmma（44.4% ） was relatively lower than in NF- Ⅰ （70.0%）, with no significant difference between them. Conclusion As a suppressing gene of apoptosis, bd-2 are closely concerned with happenings and devdopment of solitary neurofibmma and NF-Ⅰ . High expression of bcl-2 could suppress the apoptosis of turnout roll, and extend the life cycle of the cell. It also could cause the continuous growth and recurrence. There are positive expression of mtp53 in solitary neurofibmma and NF- Ⅰ, it supports the assumption that mtp53 could transfer cell, suppress apoptosis, prompt the growth of turnout cell.

关 键 词：bel一2 P53突变 Ⅰ型神经纤维瘤病 免疫组化链霉菌抗生素蛋白-过氧化酶连接法 

分 类 号：R739.41[医药卫生—肿瘤]