层黏连蛋白和纤维连接蛋白对传代人晶状体上皮细胞生长和Cx43表达的影响  

The Effects of laminin and fibronectin on human lens epithelial cells subculture characters and Cx43 expression

作　　者：王欣玲[1] 张国刚[2] 于韬[3] 阎启昌[1] 张劲松[1] 

机构地区：[1]中国医科大学附属第四医院眼科,中国辽宁省沈阳市110005 [2]沈阳药科大学中药学院,中国辽宁省沈阳市110016 [3]辽宁省肿瘤医院医学影像科,中国辽宁省沈阳市110042

出　　处：《国际眼科杂志》2006年第6期1283-1286,共4页International Eye Science

基　　金：国务院第33批博士后科学基金资助项目(No.2003033282);辽宁省教育厅高等学校科学研究项目(No.2004C041);辽宁省教育厅高等学校科学技术研究项目(No.05L576);辽宁省博士启动;自然科学基金项目(No.20062091)。~~

摘　　要：目的:观察层黏连蛋白(Ln)和纤维连接蛋白(Fn)对传代培养的人晶状体上皮细胞(hLECs)形态学和连接蛋白43(Cx43)表达的影响。方法:以层黏连蛋白(Ln)和纤维连接蛋白(Fn)处理培养皿表面,以倒置显微镜观察第3代培养的hLECs生长的形态,MTT法记录其生长曲线,免疫荧光法检测Cx43表达,比较两组表达阳性率的变化。结果:在层黏连蛋白处理组,传代细胞以典型的六角形上皮细胞形态为主,而纤维连接蛋白处理组则表现为以梭形的成纤维细胞为主。MTT实验显示,传代后4～7d纤维连接蛋白处理组细胞数多于层黏连蛋白处理组(P<0.05)。免疫荧光反应中,两组Cx43表达的阳性率有明显差别(23/28vs16/28,P<0.05)。结论:层黏连蛋白可以提供适宜hLECs保持上皮细胞状态的体外培养微环境,而纤维连接蛋白可以促进hLECs的增殖和分化。AIM： To observe the morphologic characters and Cx43 expression of human lens epithelial cells （hLECs） subcultured on laminin and fibronectin.METHODS： Inverse microscopy was applied to observe the feature of the 3rd passage hLECs secondarily cultured on laminin or fibronectin treated plates. MTT assay was used to show the growth curve of subcultured cells. Immunofluorescent staining was used to demonstrate the positive Cx43 expression. The ratio of Cx43 positive expression in the two groups were calculated and compared. RESULTS： The hLECs maintained typical morphological fea- tures in laminin group, while almost all the hLECs transformed into fibroblastic shape in fibronedin-treated group. There were more viable cells in the fibronedin group from the 4th to the 7th day by MTT reaction （P〈0.05）. Immunofluorescent staining showed significant difference between the two groups in Cx43 positive expression （23/28 vs 16/28, P〈0.05）.CONCLUSION： Laminin provide the suitable microenvironment for the hLECs to maintain the typical features of epithelia, while fibronectin promote the hLECs＇ proliferation and differentiation.

关 键 词：层黏连蛋白 纤连蛋白类 晶状体 上皮细胞 连接蛋白 

分 类 号：R776.1[医药卫生—眼科]