PTEN和p53在骨肉瘤中的表达及其临床意义  

Study of PTEN and p53 expression in osteosarcoma

作　　者：仲江波[1] 高聿同[2] 房清敏[1] 张伟[1] 孙兆忠[1] 张新军[1] 

机构地区：[1]滨州医学院附属医院脊柱外科,山东滨州256603 [2]山东省立医院骨病科

出　　处：《中国骨肿瘤骨病》2006年第6期360-363,共4页Chinse Journal Of Bone Tumor And Bone Disease

摘　　要：目的研究骨肉瘤组织中PTEN,p53的表达及其相关性,探讨其在骨肉瘤发生发展中的作用。方法用S-P免疫组化方法检测PTEN和P53在40例骨肉瘤及20例骨软骨瘤中的表达。结果PTEN在骨良性肿瘤组织中阳性表达率为90.0%,显著高于骨肉瘤组织的55.0%(P<0.05);p53在骨良性肿瘤组织中不表达,在骨肉瘤组织中阳性表达率为57.5%,差异有显著性(P<0.05);骨肉瘤组织中,PTEN和p53的表达呈负相关(r=-0.3710,P=0.0189)。PTEN的表达与骨肉瘤分化程度有关,与肺转移无关。p53的表达与骨肉瘤分化程度及肺转移无关。结论PTEN和p53与骨肉瘤的发生有关,且两种指标具有相关性,临床上联合检测PTEN和p53的表达对骨肉瘤的早期诊断具有指导意义。PTEN检测对判断骨肉瘤的预后具有指导意义。Objective To analyze expressions and correlation of PTEN and p53 in osteosarcoma and to investigate the roles of these genes in development and progression of Osteosarcoma. Methods PTEN and P53 were detected by S-P immunohistochemiscal method in 40 cases of paraffin-embedded osteosarcoma sections and 20 osteochondroma cases. Results The rate of PTEN expression was 90.0% in osteochondroma and only 55.0% in osteosarcoma tissues. A significant difference was observed between the two groups （P 〈 0.05）. The positive rate of p53 in the nucleus of osteosarcoma was 57.50% and there were no expressions in osteochondroma. A significant difference was found between them （P〈0.05）. The expressions of PTEN had negative correlation with p53 in osteosarcoma （r = -0.3710）. PTEN expression was related with pulmonary metastasis instead of tumor cell differentiation and P53 did not related with both cell differentiation and pulmonary metastasis in Osteosarcoma. Conclusions PTEN and p53 may play an important role in carcinogenesis of osteosarcoma The detection might be a guide for early diagnosis of osteosarcoma. The detection of PTEN might be a role for determinant the prognosis of osteosarcoma.

关 键 词：骨肉瘤 PTEN P53 免疫组织化学 

分 类 号：R738[医药卫生—肿瘤]