微生物转化法合成天麻素  

Synthesis of gastrodin by microbial transformation

作　　者：朱宏莉[1] 宋纪蓉[2] 黄建新[1] 张嘉[1] 马震宇[1] 杨明琰[2] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]西北大学化工学院,陕西西安710069

出　　处：《药学学报》2006年第11期1074-1077,共4页Acta Pharmaceutica Sinica

基　　金：陕西省科委科技攻关计划资助项目(社发:中药现代化)2005K16-G7(3);陕西省教育厅专项资助项目(06JK189);西北大学校内基金资助项目(04NW38)

摘　　要：目的以对羟基苯甲醛为底物,通过微生物的转化作用生物合成天麻素。方法采用静息细胞转化法并结合TLC和HPLC分析,从几十株霉菌和细菌中筛选出了一株能够转化合成天麻素的华根霉(Rhizopus chinensisStaitoAS3.1165),在优化转化条件的基础上,进行批量扩大试验,经色谱分离得到转化产物,通过理化性质和波谱分析鉴定其结构。结果经NMR及EI-MS等的测定,证明所得转化产物为天麻素。结论利用微生物对天麻素前体———对羟基苯甲醛进行选择性的还原和糖基化,从而高效率的获得天麻素,此方法的建立为天麻素的合成开辟了一条新的途径,具有较大的经济和社会价值。Aim To synthesize gastrodin via biotransformation of microorganism using phydroxybenzaldehyde as substrate. Methods Using resting cell techniques with TLC and HPLC analysis, a strain of Rhizopus chinensis Staito AS3. 1165 which has the capability of biotransforming phydroxybenzaldehyde into gastrodin was screened from 50 strains of microorganisms. Preparative scale biotransformation was carried out under the optimal transforming condition. The product was obtained by chromatography, and characterized on the basis of its physical and spectral data. Results The product was identified as gastrodin on the basis of its ^1H NMR, ^13C NMR and El-MS spectral data. Conclusion The fact that gastrodin could be synthesized by microbial transformation implies a new approach to gastrodin production. As a result, this research will be of a great economic and social value.

关 键 词：微生物转化 对羟基苯甲醛 天麻素 

分 类 号：Q81[生物学—生物工程]