多克隆CNTF抗体对肉毒毒素引起肌麻痹超微结构的影响  

The Effects of Polyclonal Antibodies against CNTF on Ultrastructure of Paralytic Muscle Induced by Botulinum Toxin

作　　者：闫丽娟[1] 吴晓[1] 孙异临[2] 李鑫[1] 

机构地区：[1]首都医科大学附属北京同仁医院眼科中心 [2]北京市神经外科研究所

出　　处：《首都医科大学学报》2006年第6期781-784,共4页Journal of Capital Medical University

摘　　要：目的研究多克隆睫状神经营养因子(c iliary neurotroph ic factor,CNTF)抗体对肉毒毒素(botu linum toxin,BTX)引起的肌麻痹的作用。方法10只新西兰兔,分别在双侧眼外肌肌腹和面部皮下注射A型肉毒毒素(botu linum toxin A,BTXA),注射后第4天在右侧眼外肌和面部皮下注射多克隆CNTF抗体,左侧相同部位注射等量生理盐水作为对照,在注射后14 d,行电镜观察双侧眼外肌和面肌的肌细胞、运动终板及神经纤维的改变。结果注射BTXA后14 d眼外肌和面肌的肌纤维均明显萎缩,线粒体肿胀,肌纤维结构无明显破坏;神经髓鞘板层松散,可见髓样小体,运动终板处可见清亮的兴奋性神经递质小泡增多密集。右侧注射CNTF组可见肌细胞核内移,胞质内线粒体肿胀聚集,肌细胞呈空泡样改变,局部肌丝断裂溶解,变性的肌细胞坏死崩解成细胞碎片;运动终板处也可见大量清亮小泡聚集,神经髓鞘增多。结论注射多克隆CNTF抗体后,与单纯注射BTXA者比较,局部肌细胞结构出现不可逆性破坏,延长了肌肉麻痹恢复时间,提示多克隆CNTF抗体可能延长BTX的肌麻痹作用。Objective The aim of this research is to study the effects of polyclonal antibodies against ciliary neurotrophic factor （CNTF） on ultrastructure of paralytic muscle induced by botulinum toxin. Methods Botulinum toxin A （BTXA）was injected into bilateral extraocular muscles and facial muscles of 10 New Zealand rabbits. After 3 days, right extraocular muscles and facial muscles were injected polyclonal antibodies against CNTF（ anti-CNTF）, while left side were injected equivalent normal saline at the same sites. After 14 d of injection BTXA, observe changes of bilateral muscle cell, motor endplate, and nerve fiber by electron microscope. Results After 14 d of injection BTXA, both extraocular muscle and facial muscle atrophy, mitochondria swell, the layers of myelin sheath loosen, seeing myelin-like body, motor end plate is full of lots of clear excitatory neurotransmitter vesicles. The group of injection anti- CNTF shows the nucleus of myocytes ingression, mitochondria swelling in endochylema, which leads to the vacuole-like degeneration of muscles, local myofilament disrupting and dissolving, degenerative myocytes necrosis and disintegrating fragments. In the motor end plate, there are lots of clear aggregating vesicles too, myelin sheaths increase. There are not obvious structure changes between both of them. Conclusion After injection of polyclonal anti-CNTF, local structure of muscle cell shows unreversible destroy rather than single injecting BTXA, which maybe prolong the time of paralytic muscle resuming. It is suggested polyclonal antibodies against CNTF may prolong the duration of muscle paralyzing induced by botulinum.

关 键 词：A型肉毒毒素 睫状神经营养因子 运动终板 眼外肌 面肌 

分 类 号：R777.4[医药卫生—眼科]