B-myb在人肝细胞癌中的表达及其临床意义  

Expression and Clinical Significance of B-myb in Hepatocellular Carcinoma

作　　者：曹骥[1] 杨春[1] 李丽萍[1] 欧超[1] 潘南海[2] 段小娴[1] 李瑗[1] 苏建家[1] 岳惠芬[1] 吴华 

机构地区：[1]广西医科大学附属肿瘤医院广西医科大学医学科学实验中心,广西南宁530021 [2]广西医科大学计算机教研室,广西南宁530021 [3]南宁市硕博生物技术有限责任公司,广西南宁530021

出　　处：《中华肿瘤防治杂志》2006年第20期1575-1577,1590,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：广西青年科学基金(桂科青0447050);广西留学回国人员科技活动择优资助项目(桂人函[2005]321号)

摘　　要：目的:探讨B-myb的mRNA在人肝细胞癌(hepatocellularcarcinoma,HCC)组织中的表达及其意义。方法:用荧光实时定量聚合酶链反应(RealtimePCR)技术检测70例HCC患者癌组织、癌旁肝组织及18例正常肝组织中B-mybmRNA的表达情况。结果:B-mybmRNA在HCC组织(0·0375±0·0168)及癌旁肝组织(0·0353±0·0128)中的表达水平明显高于在正常肝组织(0·0265±0·0099)中的表达水平,P<0·05,而在HCC组织及癌旁肝组织中的表达水平差异无统计学意义,P>0·05。B-myb在人HCC组织中的表达与临床分期、肝外转移及术后复发明显有关,而与门静脉癌栓、肿瘤个数、肿瘤直径、血清AFP水平及分化程度无明显关系。结论:B-myb可能与HCC的发生、发展有关。OBJECTIVE：To explore the expression and significance of the B-myb mRNA in human HCC （hepatocellular carcinoma） tissues. METHODS： Real time polymerase chain reaction （Real time PCR） was employed to determine the expression of B-myb mRNA in 70 HCC tissues and their adjacent liver tissues and 18 normal liver tissues. RESULTS： The expression level of B-myb mRNA in the HCC tissue （0. 037 5±0. 016 8） and the adjacent liver tissue （0. 035 3±0. 012 8） was markedly higher than in the normal liver tissues （0.026 5± 0. 009 9, P〈0. 05）. The expression level of B-myb mRNA were not significantly different in the HCC tissue than in the adjacent liver tissue （P〉0.05）. The expression of B-myb in the HCC tissue was significantly correlated with the clinical stage, the presence of extrahepatic metastasis, and the recurrence of tumor, but not correlated with the Portal Vein Tumor Thrombus, the number of tumor, the diameter of tumor, the level of serum alph-fetoprotein （AFP）, and the differentiation of tumor. CONCLUSIONS： The results suggest that B-myb may be related to the initiation and progression of HCC.

关 键 词：癌基因 基因表达 癌 肝细胞 聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤]