呼吸道合胞病毒感染对豚鼠肺组织β、Μ受体的影响  

Effects of respiratory syncytial virus infection on β-Μ-receptor density and function in lung tissue of guinea-pig

作　　者：田曼[1] 赵德育[1] 葛传生[1] 李桦[2] 郑仕忠[3] 

机构地区：[1]南京医科大学附属南京市儿童医院呼吸科,210008 [2]南京医科大学附属南京市儿童医院中心实验室,210008 [3]南京医科大学同位素室

出　　处：《中华实验和临床病毒学杂志》2006年第4期367-369,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：江苏省教委自然科学基金资助(99KJD320017;01KJD320005)

摘　　要：目的 研究呼吸道合胞病毒（RSV）感染豚鼠后肺组织β肾上腺素能受体（β受体）和M胆碱能受体（M受体）的变化。方法 应用放射性配基结合分析法，测定病毒接种组和对照组豚鼠肺组织β、M受体密度和亲和力的变化。结果 ①RSV接种后豚鼠肺组织均出现毛细支气管炎、肺炎的病理改变。②β受体最大结合容量（Bmax）在RSV接种后无明显改变。β受体平衡解离常数（Kd）在RSV接种后3d增高，5～7d达高峰，以后逐渐下降，接种后14、21 d与对照组比较差异无统计学意义。③高亲和力M受体亚型的Bmax和Kd值在感染前后无明显变化。低亲和力M受体亚型在RSV接种后3d出现Bmax增高，Kd值降低，5—7d达高峰，第21天与对照组差异有统计学意义。结论RSV感染可使β受体亲和力下降，低亲和力M受体密度增加、亲和力上升，两种受体系统之间的平衡失调是引起气道高反应性的机制之一。Objective To investigate the effects of respiratory syncytial vires （RSV） infection on β-M- receptor density and function in lung tissue of guinea-pig. Methods After intranasal inoculation of guinea-pig with RSV, the vires rapidly proliferated in lung tissue. By using the method of specific binding of isotopic ligand to β-M- receptor,the density and affinity of β-M-receptor in lung tissue were measured on days 3,5,7,9,11,14 and 21 after inoculation. Results （ 1 ） The RSV-inoculated group showed pathological evidence of acute bronchiolitis on the 3^rd day after inoculation,which reached the peak during the days 5 to 7 and recovered on day 14. （2） Viresinfection had no significant effect on the maximum binding capacity（Bmax） of β-adrenoceptor in lung tissue, and the dissociation constant of β-adrenoceptor was increased on day 3 postinoculation with RSV, peaked on day 5 to 7, which subsided by day 14,21. （3） Vires-infection had no significant effect on the B max and the Kd of the highavidity subgroup of M-cholinergic receptor. But the B max and the Kd of the low-avidity subgroup of M-cholinergic receptor appeared to increase and decrease on day 3 respectively, which reached the peak on day 5 to 7 and still showed high difference on day 21 .Conclusion The RSV infection could change the density and the affinity of β- M-receptor,which may play a role in airway hyperreactivity.

关 键 词：呼吸道合胞病毒 豚鼠 受体 肾上腺素能β 受体 胆碱能 

分 类 号：R361[医药卫生—病理学]