丝裂霉素C联合C_2-神经酰胺对人膀胱癌T_(24)细胞生长的抑制作用  

Inhibition effect of combining mitomycin C with C_2-ceramide on human bladder cancer T_24 cells

作　　者：时京[1] 孔垂泽[1] 孙志熙[1] 张莹[2] 刘博[1] 刘奔[1] 

机构地区：[1]中国医科大学附属第一医院泌尿外科,辽宁沈阳110001 [2]中国医科大学实验技术中心,辽宁沈阳110001

出　　处：《中华肿瘤防治杂志》2006年第19期1461-1463,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：辽宁省自然科学基金(20042082)

摘　　要：目的探讨丝裂霉素C(mitomycinC,MMC)与C2-神经酰胺(C2-ceramide,C2-cer)联合应用对人膀胱癌T24细胞生长的抑制作用。方法采用四甲基偶氮唑蓝[3-(4,5-dimethylthiazole-2yl)-2,5-diphenylte-trazoliumbromid,MTT]比色法测定MMC和C2-cer单独或联合应用对人膀胱癌T24细胞生长的抑制作用,并应用Chou-Tala-lay联合指数法定量分析药物之间相互作用的效果。结果MMC、C2-cer单独应用时,随药物浓度增加对T24细胞生长的抑制作用也增加,中效浓度分别为153.426、27.797μmol/L。MMC与C2-cer联合应用时,相互作用的效果表现为在低浓度(0<fa<43%)时呈协同作用(CI<1),高浓度(43%<fa<100%)时呈拮抗作用(CI>1),中效浓度为89.536μmol/L(其中MMC74.048μmol/L,C2-cer15.488μmol/L)。结论MMC和C2-cer相互作用的效果与药物浓度相关,低浓度时呈协同作用,高浓度时呈拮抗作用。OBJECTIVE： To explore the anti-cancer effects.of mitomycin C （MMC） and C2-ceramide （C2-cer） on human bladder cell line T24. METHODS： The MTT method was used to detect the inhibition rate and the combination index method of Chou and Talalay was used to quantitatively assay the effect of combined application. RESULTS： The anti-cancer effects of MMC and C2-cer were enhanced with the increased drug concentrations when they were used alone. The respective median-effect concentrations were 153. 426 and 27. 797 μmol/L. MMC was synergetic with C2-Cer （CI〈1） at low concentrations （0%〈fa 〈43%） and was antagonist with C2-Cer （CI〉1） at high concentrations （43 % 〈 fa〈 100 % ）. The median-effect concentration was 89. 536 μmol/L （MMC 74. 048 μmol/L, C2-cer 15. 488 μmol/L）. CONCLUSIONS： The effects of combination between MMC and C2-cer are relevant to their concentrations. MMC is synergistic with C2-cer at lower concentrations and antagonistic at higher concentrations.

关 键 词：丝裂霉素C C2-神经酰胺 膀胱肿瘤/药物疗法 

分 类 号：R737.14[医药卫生—肿瘤]