磁性纳米控释紫杉醇对食管癌Eca109细胞株生长的影响  

Effects of magnetic nano-controlled release paclitaxel on the growth of esophageal carcinoma cell line Eca109

作　　者：陈萍[1] 施瑞华[1] 凌亭生[1] 

机构地区：[1]南京医科大学第一附属医院消化内科,江苏省南京市210029

出　　处：《世界华人消化杂志》2006年第35期3348-3352,共5页World Chinese Journal of Digestology

基　　金：江苏省卫生厅资助项目;No.2001-31~~

摘　　要：目的:观察不同浓度磁性纳米控释紫杉醇对食管癌细胞Eca109的影响及与不同化疗药物的对比.方法:取对数生长期的食管癌Eca109细胞作细胞增殖抑制实验,实验组分别给予不同浓度的磁性纳米控释紫杉醇和5-氟尿嘧啶、泰素,同时设立二甲基亚砜(DMSO)和RPMI1640液对照,测定24,48,72h三个时间段的吸光度值,计算抑制率.经不同浓度的磁性纳米控释紫杉醇作用72h后,用电镜观察细胞超微结构,同时用流式细胞仪测定细胞周期和细胞凋亡.结果:MTT实验显示磁性纳米控释紫杉醇可抑制食管癌细胞增殖,与5-氟尿嘧啶,泰素相比,具有缓释性(P<0.01);电镜可发现药物作用组细胞核固缩、解聚以及凋亡小体;流式细胞仪检测显示G1峰前有明显的凋亡峰;细胞周期分析提示磁性纳米控释紫杉醇可将Eca109细胞阻滞于G2-M期,且与浓度相关.结论:磁性纳米控释紫杉醇对人食管癌细胞Eca109的生长有明显的抑制作用,使细胞分裂阻滞于G2-M期,并诱导细胞凋亡,且具有缓释效果.AIM： To observe the effects of magnetic nano- controlled paclitaxel at different concentrations on the growth of esophageal carcinoma cell line Eca109. METHODS： Eca109 cells in logarithmic growth phase were treated by magnetic nano-controlled release paclitaxel at different concentrations, fluorouracil （5-FU） and Taisu, while dimethylsurfoxide （DMSO） and RPMI 1640 were used in the control cells. MTT assay was used to examine the inhibitory rate through the absorbency of 24, 48, and 72 h. After Ecal09 cells were disposed by magnetic nano-controlled release paclitaxel at different concentrations for 72 h, the cellular ultrastructure was observed under electron microscope and cell cycle and apoptosis were analyzed by flow cytometry. RESULTS： Magnetic nano-controlled release paclitaxel inhibited the proliferation of Eca109 cell in vitro, and the effect had characteristic of slower release in comparison with that of 5-FU or Taisu （78.1% vs 60.0%, 63.8%, P 〈 0.01）. Chromation condensation, nucleic fragmentation and apoptic body formation were found in drug-treated cells by electron microscopy. Flow cytometry analysis showed an obvious apoptotic peak before G1 peak and magnetic nanocontrolled release paclitaxel blocked Eca109 cells in G2-M phase, which was associated with the concentrations. CONCLUSION： Magnetic nano-controlled release paclitaxel can induce apoptosis and block cell cycle of esophageal cells.

关 键 词：磁性纳米控释紫杉醇 食管肿瘤 细胞周期 细胞凋亡 

分 类 号：R735.1[医药卫生—肿瘤]