大鼠急性β射线皮肤损伤动物模型的建立与应用  

Establishment and application of rat model of acute β-irradiated skin injury

作　　者：沈国良[1] 陆兴安[1] 唐俊[1] 王修珍[2] 吴士良[2] 田野[2] 

机构地区：[1]苏州大学附属第一医院烧伤整形外科,215006 [2]苏州大学基础医学部

出　　处：《中华放射医学与防护杂志》2006年第6期577-579,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国防基础科研计划基金资助项目(2003044)

摘　　要：目的建立大鼠急性β射线皮肤损伤动物模型,并用此模型研究超氧化物歧化酶(SOD)对创面愈合作用。方法雄性SD大鼠40只,随机分为2组,每组20只。治疗组:45Gyβ射线照射SOD治疗;对照组:45Gyβ射线照射生理盐水对照。采用直线加速器产生的β射线照射大鼠臀部皮肤20mm×40mm。观察创面出现及愈合的时间。光镜及免疫组织化学检查β射线皮肤损伤创面愈合过程中组织学改变及血管内皮细胞生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)表达的变化。结果45Gyβ射线照射后出现典型深Ⅱ度烧伤创面。SOD治疗组创面愈合速度明显比生理盐水对照组快(P<0·05)。β射线照射后第6、7、8周时,治疗组与对照组免疫组织化学VEGF、bFGF表达分别为强阳性和阳性。β射线照射后第4、5、9周时,2组表达均为弱阳性。结论采用直线加速器产生β射线照射建立急性β射线皮肤损伤动物模型简便、快速,剂量准确、可靠,是研究外用药促进急性β射线皮肤损伤创面愈合的较理想动物模型,SOD具有促进急性β射线皮肤损伤创面愈合的作用。Objective To establish an experimental rat model of acute β-irradiated skin injury and to study the effects of superoxide dismutase （SOD） on wound heating. Methods Areas of buttock skin （20 mm × 40 mm） of 40 male SD rats were irradiated with 45 Gy β-rays generated by linear accelerator, and then the forty rats were divided into two groups randomly： treatment group administrated with SOD （n = 20） and control group administrated with normal saline （NS） （n = 20 ）. The wound healing time and rate were observed. The pathological changes were observed by light microscopy. The expressions of VEGF （vascular endothelial growth factor） and bFGF （basic fibroblast growth factor） were determined by SP immunohistochemical method. Results The deep second-degree burns was observed following 45 Gy irradiation. The wound healing time in treatment group was shorter than that of the control group （ P 〈 0.05）. Strongly positive （ ＋＋＋ ） expression of VEGF, bFGF in treatment group and positive （ ＋ ＋ ） expression of VEGF, bFGF in the control group were observed 6 weeks, 7 weeks and 8 weeks after the irradiation, while only weakly positive （ ＋ ） expressions of VEGF and bFGF in both groups 4 weeks, 5 weeks and 9 weeks after the irradiation. Conclusions The wound model of acute β-irradiated skin injury in rat was established and used in study of the effect of medicine on wound healing. SOD can promote the wound healing of acute β-irradiated skin injury.

关 键 词：Β射线 放射性皮肤损伤 创面愈合 动物模型 超氧化物歧化酶 

分 类 号：R-332[医药卫生]