大蒜素逆转内毒素致内皮细胞损伤的实验研究  

Reverting Effects of Allitridi on Endotoxin-induced Endothelial Cell Injury

作　　者：林春霞[1] 王瑞英[2] 曹竹龄[2] 郝爱荣[3] 王晶[3] 

机构地区：[1]中国协和医科大学阜外心血管病医院中德分子实验室,100037 [2]武汉大学医学院中南医院心内科,430071 [3]武汉大学医学院第一附属医院心内科,430071

出　　处：《中国分子心脏病学杂志》2002年第2期67-71,共5页Molecular Cardiology of China

摘　　要：观察大蒜素能否体外逆转/改善内毒素致内皮细胞损伤并探讨其可能机制。方法 将人脐静脉内皮细胞系ECV304与浓度为100μg/ml的内毒素共同孵育,并用不同浓度的大蒜素加以处理,实验共分三组:(1)空白对照组(加用无血清培养基,n=6);(2)内毒素对照组(加含内毒素的无血清培养基,c=100μg/ml,n=6);(3)大蒜素+内毒素组(加含内毒素为100μg/ml的无血清培养基,大蒜素浓度分别为10、20、50μg/ml,n=6)。选用氧化抗氧化,内皮舒张功能及增殖方面的指标研究不同处理因素对内皮细胞的功能影响。结果 1.大蒜素+内毒素组培养基中NO_2^-、NO_3^-含量较内毒素对照组显著降低(P<0.01),较空白对照组显著增高(P<0.01或0.05),且效应呈大蒜素剂量依赖性递减,时间依赖性递增。2.大蒜素+内毒素组内皮细胞的T-SOD活力较内毒素对照组显著增高(P<0.01),并呈时间剂量依赖性,大蒜素50μg/ml组较空白对照组显著升高(P<0.01)。3.大蒜素+内毒素组培养基中MDA含量较内毒素对照组显著降低(P<0.01),较空白对照组仍显著升高(P<0.01),且效应呈大蒜素剂量依赖性。4.大蒜素+内毒素组较内毒素对照组能促进内皮细胞增殖(P<0.05),HSP_(70)的表达显著增高(P<0.01)结论 大蒜素能够逆转做善内毒素致内皮细胞损伤,对防治内毒素参与AS发病方面具有积极作用?Objectives To observe the reverting effects of allitridi on the endotoxin-indueed endothelial cell injury and its possible mechanism. Methods Human umbilical venous endothelial cells （HUVEC） ECV304 were treated by different doses of allitridi after co-cuhivated at the same dose of endotoxin. The experiment was divided into 3 groups ： （ 1 ）blank control group （non-serum media without drug, n = 6） ; （2） endoloxin control group （ non-serum media with endotoxin, e = 100μg/ml） ; （3） allitridi ＋ endotoxin groups （non-serum media with the same dose of endotoxin and different doses of allitridi, e 10, 20,50μg/ ml,n=6）. Results Compared to endotoxin control group, in allitridi ＋ endotoxin group, 1. the NO2^-, NO3^- contents groups were dose-dependently decreased and timedependently increased ; 2. The T-SOD ae tivity of endothelial cell was significantly increased, while the MDA contents were dramatically decreased and both were in a dose-dependent manner （all P〈0.01 ） ; 3. The proliferation of EC were promoted （all P〈0.05） and endothelial cell were in a normal morphology; 4. The expression of HSP70 was remarkably increased （all P〈0.01）.

关 键 词：大蒜素 内皮细胞 损伤 动脉粥样硬化 

分 类 号：R54[医药卫生—心血管疾病]