香蕉汁对花生组织培养及抗氧化酶活性的影响  

Effect of Banana Juice on Tissue Culture of Peanut and Activity of Antioxidase

作　　者：刘宾[1] 蒋继志[1] 廖祥儒[1] 崔哲[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2006年第6期631-635,共5页Journal of Hebei University(Natural Science Edition)

基　　金：河北省自然科学基金资助项目(300081);河北省省级重点学科生物工程资助项目

摘　　要：通过对花生上胚轴组织培养,研究了香蕉汁对愈伤组织诱导的影响和外植体体内可溶性蛋白、SOD,CAT,POD,AP几个生理指标的变化及其与愈伤组织诱导的关系.结果表明:接种在香蕉汁培养基中的上胚轴外植体的愈伤组织诱导率最高,达95%;香蕉汁使外植体愈伤组织的蛋白质含量提高,SOD的活性受到抑制,CAT,AP和POD的活性被增强.Effect of banana juice on tissue culture of peanut epicotyl and changes of protein content and activity of SOD,CAT, POD,AP in callus were investigated in this experiment. The results showed that the media with banana juice was optimum for callus induction of peanut epicotyl, which induction rate was 95 percent; The protein content in the explant callus was raised, the activity of SOD was inhibited, while the activity of CAT, POD, and AP are strengthened to some extent.

关 键 词：花生 上胚轴 香蕉汁 抗氧化酶 

分 类 号：Q945[生物学—植物学]