14肽化合物抗脂肪肝作用的实验研究  

Effects of polypeptide compound (CMSO14) on fatty liver in rats

作　　者：张磊 付洪[2] 辜岷[2] 金爱琼[2] 吴谦[3] 

机构地区：[1]深圳市康哲药业有限公司,广东深圳518029 [2]深圳市第二人民医院 [3]广东省药物研究所

出　　处：《中西医结合肝病杂志》2006年第6期350-351,353,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:观察14肽类化合物CMS014对大鼠酒精性肝损伤(脂肪肝)的防护作用。方法:以10%酒精加高营养喂养6周造成大鼠脂肪肝后分组处理,给药组皮下注射CMS014,剂量为1.5mg/kg.d-1(低剂量组)或4.5mg/kg.d-1(高剂量组),疗程4周,另设正常对照组和造模对照组。实验结束时取血测血脂、肝功能,处死动物并剖取肝脏做病理检查,制备肝组织匀浆,测定肝脂及脂质过氧化指标。结果:与造模组比较,CMS014高剂量组肝匀浆甘油三酯(88.7±18.6)mg/d l、丙二醛(49.2±13.9)nM/g显著降低(分别为P<0.001,P<0.0001),而还原型谷胱甘肽(42.2±6.3)mg/g及超氧化物歧化酶(32.4±2.0)U/g显著增高(分别为P<0.01,P<0.05);CMS014低剂量组有类似结果;高剂量组CMS014还具有降低血脂、改善肝功能作用;病理检查显示注射CMS014的两组大鼠肝细胞脂肪变性范围缩小。结论:多肽化合物CMS014对大鼠脂肪肝具有显著防护作用,其机制可能与抗脂质过氧化有关。Objective： To investigate the protective effect of polypeptide compound （CMS014） on fatty liver in rats. Methods： The model of fatty liver was induced by feeding rats with 10% alcohol plus high nutrition for 6 weeks. These rats were treated with administration （s. c） of 1.5 or4. 5mg/kg·d^-1 CMS014 for4 weeks. The normal group and model group control were also set up in the study. Blood samples were collected for the measurement of serum lipid and liver function at the end of the experiment. All rats were then killed for the hepatic pathological examination and preparation of hepatic tissue homogenate, in which the hepatic lipid and hyperoxide would be tested. Results： The high-dose CMS014 group showed remarkable lower levels of homogenate triglyceride （88. 7 ± 18.6） mg/dl and malonaldehyde （49. 2 ± 13.9） nM/g with higher levels of homogenate glutathione （42.2 ±6.3） mg/g and superoxide dismutaes （32.4 ±2.0） U/g when compared to the model control group. The similar results were seen in the low-dose CMS014 group. High-dose CMS014 also decreased the level of serum lipid and improved liver function. The pathological examination demonstrated that there was less fatty denaturation in the hepatic cells in both CMS014 groups. Conclusion： Polypeptide CMS014 has markedly protective effects on fatty liver in rats. Its mechanism may refer to anti-lipoperoxide.

关 键 词：多伏/药效学 脂肪肝 脂质过氧化 大鼠 

分 类 号：R575.5[医药卫生—消化系统]