展青霉素人工抗原及多克隆抗体的研制  

Preparation of Artificial Antigen and Polyclonal Antibody of Mycotoxin Patulin

作　　者：邓舜洲[1] 许杨[1] 

机构地区：[1]南昌大学食品科学教育部重点实验室,江西南昌330047

出　　处：《食品与生物技术学报》2006年第6期13-17,53,共6页Journal of Food Science and Biotechnology

基　　金：国家"十五"重大科技专项课题(国科农社函[2002]130号)

摘　　要：采用戊二酸酐法合成展青霉素-半戊二酸(PAT-HG),通过活泼酯法将PAT-HG偶联到牛血清白蛋白,制备了展青霉素免疫抗原(PAT-HG-BSA),免疫3只BALB/c小鼠,经4次免疫后,1号小鼠血清抗展青霉素抗体效价达1∶1600,且小鼠抗展青霉素多克隆抗体与展青霉素检测抗原的结合能被展青霉素阻断。The hemiglutarate-patulin （PAT-HG） was prepared by esterification with glutarate, then, it was conjugated to bovine serum albumen（BSA） to form immunogen （PAT-HG-BSA） by ester method. The titer of anti-PAT antibodies in one BLAB/c miee serum, immunized by PAT- HGrBSA, was about 1 ： 1600, in which antibodies binding to PAT-MOVA was inhibited by free patulin.

关 键 词：展青霉素 人工抗原 多克隆抗体 

分 类 号：TQ465.1[化学工程—制药化工]