HnRNP A2/B1短发夹环RNA重组体的构建与序列分析  

Construction and Sequence Verification of an Expression Vector for shRNA Specific for HnRNP A2/B1

作　　者：蒲丹[1] 李为民[1] 陈敏[1] 陈文彬[1] 董恒磊（校对） 

机构地区：[1]四川大学华西医院呼吸内科,成都市610041 [2]不详

出　　处：《中国肿瘤临床》2006年第24期1386-1389,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金项目(编号:30270587);教育部博士点基金资助(编号:20050610056)

摘　　要：目的:利用RNA干扰(RNAinterfering,RNAi)技术构建HnRNPA2/B1重组质粒,并行序列分析。方法:分别将21bP长短的HnRNPA2/B1靶序列,中间为7bP序列间隔的反向重复序列,置入PsiRNA-hH1neoG2质粒中,产生重组质粒PSiRNA-hH1neoG2HnRNPA2/B1。结果:将重组质粒作测序分析,经测序鉴定确定为所需序列。结论:特异性短发夹环RNA(ShRNA)真核表达载体PSiRNA-hH1neoG2HnRNPA2/B1的成功构建,为进一步研究其基因功能奠定基础。Objective：To construet a recombinant plasmid for use in RNA interference experiments on HnRNP A2/B1 and to werify its construetion with sequence analysis.Metbods：The 2IbP HnRNP A2/B1 target sequentce and the 7bP inverted repeat in the middle of the sequence were inserted in the PsiRNA-hHlneoG2 plasmid to produce the reeombinant plasmid PsiRNA-hHlneoG2 HnRNP A2/B1.Results ：Sequencing analysis was performed on the recombinant plasmd.Conclusion：Successful construction of this vector may help to find a new treatment method for various tumors.

关 键 词：HNRNPA2/B1 RNA干扰 短发夹状RNA 

分 类 号：R734.2[医药卫生—肿瘤]