人卵巢癌多细胞团簇在细胞外基质中的黏附性和扩散性  

Adhesion and Dissemination of Multicellular Aggregates on Extra Cellular Matrix in Human Ovarian Carcinoma

作　　者：刘召芬[1] 陈建利[1] 席晓薇[1] 万小平[1] 

机构地区：[1]上海交通大学第一人民医院妇产科,上海200080

出　　处：《上海交通大学学报（医学版）》2006年第12期1315-1319,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30371482);中国博士后基金(2005037141)资助项目

摘　　要：目的研究人卵巢癌多细胞团簇(MCAs)在细胞外基质(ECM)中的黏附性和扩散性。方法液体重叠培养获得MCAs。将MCAs悬液以及加入不同的细胞黏附分子(CD44、CD29、CD54、E-cad)单克隆抗体(mAb)和m IgG(作为对照)的MCAs悬液,接种至覆以ECM或牛血清蛋白(BSA)(作为对照)的96孔板,培养6 h,记录每孔0 h团簇和6 h的黏附团簇数,并计算黏附率;分别测定团簇0 h和24 h的面积,以其面积变化倍数确定团簇的扩散性。结果MCAs对ECM黏附率为38%,明显高于BSA对照组的8%(P=0.001);CD29 mAb和CD44 mAb对团簇黏附的抑制率分别为60%和29%;MCAs在ECM上24 h几乎完全扩散,面积扩大倍数明显高于BSA对照组(5.96±0.92vs1.34±0.9)(P<0.01);CD29 mAb和CD44 mAb对团簇扩散的抑制率分别为74%和33%。结论MCAs具有的黏附性和扩散性可能是卵巢癌难治的重要原因。CD29 mAb和CD44 mAb可抑制MCAs的黏附和扩散,为抗黏附治疗卵巢癌提供了新策略。Objective To study the adhesion and dissemination of multicellular aggregates （MCAs） on extra cellular matrix （ECM） in human ovarian carcinoma. Methods MCAs were generated by the liquid overlay technique. MCAs, MCAs with monoclonal antibody （mAb） of four cell adhesion molecules （CD29, CD44, CD54 and E-cad） and MCAs with mIgG （control） were incubated in serum-free media on 96-wells coated with ECM for 6 h. MCAs at 0 and 6 h were counted, respectively, and MCAs dissemination was determined as the fold change in area of MCAs from 0 to 24 h. Results The adhesion rate of MCAs to ECM was 38% , significantly higher than that of the control group （8% , P =0. 001）. CD29 mAb and CD44 mAb partially inhibited MCAs adhesion, and the rate of inhibition was 60% and 29% , respectively. MCAs were seeded and completely disaggregated on ECM, resulting in a 5.96 ±0.92 fold change in area after 24 h compared with the control group （1.34 ± 0.9, P 〈 0.01 ）. CD29 mAb and CD44 mAb significantly inhibited the dissemination, and the inhibition rate was 74% and 33% , respectively. Conclusion The adhesion and dissemination of MCAs may contribute to the difficulties in treating ovarian carcinoma. CD29 and CD44 play an important role in facilitating ovarian cancer invasion and dissemination, indicating that ovarian carcinoma may be well suited for CD29 mAb and CD44 mAb mediated anti-adhesion therapy.

关 键 词：卵巢癌 多细胞团簇 细胞黏附分子 黏附 扩散 

分 类 号：R737.31[医药卫生—肿瘤]