塞来昔布对人子宫内膜腺癌细胞系HEC-1B增殖和凋亡的影响  

Effects of Celecoxib on Proliferation and Apoptosis of Human Endometrial Adenocarcinoma Cell Line HEC-1B

作　　者：肖义涛[1] 罗来敏[1] 张睿[1] 

机构地区：[1]上海交通大学第六人民医院妇产科,上海200233

出　　处：《上海交通大学学报（医学版）》2006年第12期1343-1346,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市科委基金(04JC14064);上海市卫生局基金(2005ZD006)资助项目

摘　　要：目的探讨环氧化酶-2（COX-2）选择性抑制剂塞来昔布对人子宫内膜腺癌细胞系HEC-1B增殖及凋亡的影响及其作用机制。方法噻唑蓝（MTT）法检测不同浓度塞来昔布作用HEC-1B细胞后细胞增殖的变化；不同浓度塞来昔布作用HEC-1B细胞24h后，流式细胞仪检测细胞周期和凋亡；RT—PCR检测细胞中COX-2 mRNA的表达。结果MTT结果显示．塞来昔布对HEC-1B细胞的增殖抑制作用呈现时间依赖性和浓度依赖性；不同浓度塞来昔布作用HEC-1B细胞24h后，流式细胞仪结果显示G0／G1期细胞增加，S期和G2／M细胞减少，细胞凋亡率增加，各药物组与对照组相比差异均有统计学意义（P〈0．05）；RT—PCR结果显示，在50～200μmol／L塞来昔布组，随着药物浓度的增加COX-2 mRNA的表达逐渐减弱（P〈0．05）。结论塞来昔布能抑制HEC-1B细胞的增殖，并能改变细胞周期和诱导凋亡，其机制可能与下调细胞中COX-2 mRNA的表达有关。Objective To study the effects of cyclooxygenase-2 （ COX-2 ） selective inhibitor celecoxib on proliferation and apoptosis of human endometrial adenocarcinoma HEC-1B cell line. Methods Methabenzthiazuron （MTT） assay was used to examine the effects of different concentrations of celecoxib on proliferation of HEC-1B cells. When HEC-1B cells were treated with different concentrations of celecoxib for 24 h, cell cycle and apoptosis were detected by flow cytometry. The expression of COX-2 mRNA was analyzed by RT-PCR. Results MTT results indicated that celecoxib could inhibit HEC-1 B cell proliferation in a time- and dose-dependent manner. When HEC-1B cells were treated with different concentrations of celecoxib for 24 h, flow cytometry results showed that the cell percent of G0/G1 phase increased, S and G2/M phase decreased, and cell apoptosis rate also increased, which was significantly different from that of the control group （ P 〈 0.05 ）. The results of RT-PCR indicated that the expression of COX-2 mRNA gradually decreased with the increasing dose of celecoxib （ 50 - 200 μmol/L） （ P 〈 0.05 ）. Conclusion Celecoxib can inhibit the proliferation of HEC-1B cells, alter cell cycle and induce cell apoptosis. The mechanism might be associated with the down-regulation of COX-2 mRNA expression in HEC-1B cells.

关 键 词：环氧化酶-2 塞来昔布 子宫内膜腺癌 增殖 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]