贵州省同一产地野生和栽培天麻的RAPD分析  

RAPD Analysis on Wild and Cultivated Gastrodia elata Blume from the Same Habitat in Guizhou Province

作　　者：邹佳宁[1] 宋聚先[1] 常楚瑞[2] 王晓丽[2] 

机构地区：[1]贵阳医学院分子生药学实验室,贵州贵阳550004 [2]贵阳医学院药用植物学与生药学教研室,贵州贵阳550004

出　　处：《贵阳医学院学报》2006年第6期514-516,共3页Journal of Guiyang Medical College

基　　金：科技部2004BA721A33项目;贵州省科技黔科合农社字(2001)1125号项目;黔科合中药专字[2003]51号项目

摘　　要：目的：对贵州省同一产地野生和栽培天麻的遗传多态性进行分析。方法：采用随机扩增多态性DNA标记技术。结果：从30个随机引物中筛选到6个多态性强、重复性好的引物。该6个引物的扩增结果表明，贵州省同一产地野生和栽培天麻的指纹图谱有明显差异，差异系数为40％～60％，而栽培天麻之间的差异并不显著。结论：本实验为天麻的分子生物学鉴定及天麻种质资源合理利用及科学的栽培育种提供了一定的科学依据。Objective： To compare wild and cultivated Gastrodia elata Blume from the same habitat in Guizhou province genetically. Methods： RAPD Molecular labelling technique was used and six primers with good polymorphism and repeatability were selected from 30 random primers Results： amplification results showed that the fingerprints of cultivated and wild samples had apparent difference, with variation coefficients between 40%-60%, while the variance among cultivated samples was very small. Conclusion： Our experiment provides basic information for molecular identification, reasonable use of genetic resources, and scientific breeding and culture of Gastrodia elata Blume.

关 键 词：天麻 随机扩增多态DNA技术 药用植物栽培 

分 类 号：R282.71[医药卫生—中药学]