白色念珠菌生物膜形成的定量分析和形态学观察  

An observation and quantitive analysis of the formation of Candida albicans biofilm

作　　者：綦成[1] 张春燕[2] 亓庆国[3] 

机构地区：[1]山东大学第二医院口腔科,山东济南250033 [2]山东省立医院口腔科,山东济南250021 [3]山东大学口腔医学院,山东济南250012

出　　处：《牙体牙髓牙周病学杂志》2006年第12期674-677,共4页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(30400498);山东大学青年科学研究项目(2002061)

摘　　要：目的:XTT法定量分析并在激光共聚焦显微镜和荧光显微镜下观察白色念珠菌生物膜的动态形成过程。方法:经过血清处理的灭菌玻片在六孔板中形成生物膜,经过荧光染色后在荧光显微镜和激光共聚焦显微镜下,动态观察生物膜的形成过程,并利用XTT减低法定量分析生物膜的形成过程。结果:形态学和定量分析都表明,白色念珠菌可以在玻片上形成典型的、成熟的生物膜结构,形成过程经过了几个明显的阶段。结论:白色念珠菌形成的生物膜具备典型的微生物生物膜结构。AIM： Candida albicans is the important opportunistic pathogens which biofilms are protected niche for C. albicans, where they are safe from antibiotic treatment and can create a source of persistent infection. Using fluorescence and confocal scanning laser microscopy , we observed the formation of Candida albicans biofilm , and analysis the biofilm formation by XTT reduction. METHODS： Candida albicans model strain ATCC 90038 formed biofilm structure on the glass plates in 6 well cell culture plates. The bioflm structure was observed by CLSM and fluorescent microscope. The quantitative analysis the of formation of biofilm was performed by XTT reduction method. RESULTS AND CONCLUSION：Canida albicans biofilm formation proceeds through three distinct developmental phases, and they can form mature and classic biofilm structure on the glass plates.

关 键 词：白色念珠菌 生物膜 XTT减低法 

分 类 号：R780.1[医药卫生—口腔医学]