铜绿假单胞菌基因型与生物被膜形成能力的分析  

Evaluation of the relationship between biofilm-Forming ability and ERIC-PCR based genotype in pseudomonas aeruginosa strains

作　　者：石磊[1] 寇娅丽[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《食品与机械》2006年第6期5-7,共3页Food and Machinery

基　　金：国家自然科学基金重点项目(项目编号:20436020);广东省自然科学基金项目(项目编号:04020050);广州市科技计划项目(项目编号:2004J1-C0161)

摘　　要：通过改良的组织平板培养法及ERIC-PCR方法对48株PA生物被膜的形成进行定量分析并绘制其指纹图谱。结果发现48株PA菌株生物被膜形成能力不同,且在17%相似水平上分为A、B、C、D、E五个基因型,其中生物被膜形成能力较弱的菌株大多属于前三个基因型,而D和E基因型则包含了大部分生物被膜形成能力较强的菌株,这表明不同生物被膜形成能力的PA在5个基因型中的分布并非随机的,菌株生物被膜形成能力和基因型之间具有一定的相关性。Biofilms have been a great problem to food safety and human health. And it is also hard to eradicate them. In this paper, we used a modified microtiter dish method and ERIC-PCR to lest the biofilm-forming ability and genotype in 48 Pseudomonas aeruginosa strains, The results showed that each Pseudomonas aeruginosa had different biofilm-fonning ability and in ERIC-PCR analysis five groups were identified at a similarity level of 17%. Most of the strains which showed higher biofilm-forming ability belonged to cluster D and E, while most others with lower ability pertained to the first three clusters. So we concluded that the strains were not randomly distributed in the five ERIC-PCR groups and there was a relationship between the biofilm-forming ability and genotype.

关 键 词：铜绿假单胞菌 生物被膜 基因型 

分 类 号：TS201.3[轻工技术与工程—食品科学]