H4亚型家养水禽流感病毒分离株的表面膜蛋白基因的序列测定和遗传进化分析  

Phylogenetic Analysis of the Surface Glycoprotein Genes of Three H4 Avian Influenza Virus Isolates from Water Fowl

作　　者：薛峰[1] 顾敏[1] 彭宜[1] 姚春峰[1] 张评浒[1] 钱忠明[1] 刘秀梵[1] 

机构地区：[1]扬州大学农业部畜禽传染病学重点开放实验室,扬州225009

出　　处：《畜牧兽医学报》2006年第12期1334-1339,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技攻关项目(2004BA519A02);江苏省属高校重大基础研究项目(05KJA23016)

摘　　要：采用常规的血清学试验和特异性RT-PCR方法对华东地区家养水禽中流感病毒的带毒状况进行4年多的监测,分离鉴定出多株H4亚型禽流感病毒。对其中的A/Duck/Yangzhou/216/2002(简称Dk/YZ/216/02)、A/Duck/Yangzhou/526/2003(简称Dk/YZ/526/03)、A/Duck/Yangzhou/36/2004(简称Dk/YZ/36/04)的血凝素基因和Dk/YZ/526/03、Dk/YZ/36/04的神经氨酸酶基因进行了克隆测序,并与GenBank中收录的其它序列进行了比较,遗传进化结果表明Dk/YZ/216/02的血凝素基因(HA)与毒株Tk/Minnesota/833/80(H4N2)同源性最高,而Dk/YZ/526/03和Dk/YZ/36/04的血凝素基因(HA)均与Budgerigar/Hokkaido/1/77(H4N6)同源性最高;而神经氨酸酶基因(NA)遗传进化分析结果表明Dk/YZ/36/04(H4N6)的NA基因与毒株Pigeon/Nanchang/8-142/2000(H3N6)同源性最高,而Dk/YZ/526/03(H4N2)的NA基因与Dk/Hokkaido/13/00(H9N2)同源性最高,3株禽流感病毒的HA推导的氨基酸剪切位点序列均为P-E-K-A-S-R,为典型低致病性禽流感病毒的特征序列,与对SPF鸡的致病力试验相吻合。Several H4 subtype avian A influenza live bird markets when we surveying the ecology viruses were isolated from waterfowls in some of the influenza in East China for more than four years and identified by specific RT-PCR. In this paper, the hemagglutinin （HA） gene and neuraminidase （NA） gene of three representative virus named A/Duck/Yangzhou/216/2002 （Dk/YZ/ 216/02）, A/Duck/Yangzhou/526/2003 （Dk/YZ/526/03） and A/Duck/Yangzhou/36/2004 （Dk/ YZ/36/04） had been sequenced and their pathogenicity to SPF chickens had been determined. The results showed that the HA nucleotide sequence of Dk/YZ/216/02 have high identity with Tk/Minnesota/833/80 （H4N2）, that of Dk/YZ/526/03 and Dk/YZ/Z36/04 have high identity with Budgerigar/Hokkaido/1/77 （H4N6）; Phylogenetic analysis of the N6 NA genes revealed that the NA gene of Dk/YZ/Z36/04 had genetically close relationships with that of H4N6 viruses isolated from pigeon in jiangxi China, the NA gene of Dk/YZ/526/03 had genetically close relationships with that of H9N2 viruses isolated from duck in Hokkaido. The sequences of cleavage site between HA1 and HA2 consist of P-E-K-A-S-R in all three AIVs, coincide with that of identical low pathogenic AIV, and none of the chickens inoculated with the three AIVs displayed any obvious signs of clinical disease.

关 键 词：禽流感病毒 H4 HA NA 剪切位点 

分 类 号：S852.659.5[农业科学—基础兽医学]