母鸡输卵管子宫部初级感觉神经元的CB-HRP法定位研究  

The Innervation and Location of the Primary Sensory Neurons on the Hens' Uterus of Uterine Tube by CB-HRP Methods

作　　者：张德禄[1] 崔燕[2] 胡春香[1] 刘永定[1] 

机构地区：[1]中国科学院水生生物研究所,武汉430072 [2]甘肃农业大学动物医学院,兰州730070

出　　处：《畜牧兽医学报》2006年第12期1360-1363,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30170112);中国科学院(KSCX-SW-332);水生所领域前沿项目(220418)

摘　　要：应用CB-HRP溶液注入母鸡输卵管子宫部浆膜下,四甲基联苯胺(TMB)组织化学呈色,以探察母鸡输卵管子宫部初级感觉神经元的定位。结果表明:母鸡输卵管子宫部的初级感觉神经元位于双侧T1-LS13脊神经节、颈静脉神经节和结状神经节。标记细胞数分布不均,在体左侧多于体右侧,在脊神经节多于颈静脉神经节和结状神经节。在脊神经节内标记细胞有T5-LS1和LS8-LS11前后两个相对集中区,峰值分别在T7和LS11。说明尽管母鸡输卵管子宫部是单侧发育成熟脏器,但其感觉沿双侧脊神经和迷走神经传入中枢,以体左侧传入为主;且感觉经脊神经传入较迷走神经传入中枢优势明显。The location and distribution of the primary sensory neurons on the hens＇ uterus of uterine tube were determined by retrograde trace method. CB-HRP solution was injected into the wall of the uterus of ten Issa-brown hens. The results showed that the primary sensory neurons of Issa-brown-hens uterus of uterine tube were innervated by bilateral spinal ganglia from T1 to LS13 ,bilateral jugular and nodose ganglia. The numbers of labeled neurons in the left were more than those in the right,and that in the spinal were more than those in the jugular and nodose ganglia. A lot of labeled neurons in the spinal ganglia were accumulated on the segments T5- LS1 and LS8-LS11. The greatest concentration of labeled neurons were in T7 and LS11. It was revealed that hens＇ uterus of uterine tube were innervated by bilateral spinal and vagus afferent nerves, in which mainly conducted by ipsilateral or left side spinal and vagus nerves,and the spinal was dominat,although the uterus of uterine tube of hens was the unilateral visceral organ.

关 键 词：母鸡 初级感觉神经元 输卵管子宫部 定位 CB-HRP 

分 类 号：S831.1[农业科学—畜牧学] S852.1[农业科学—畜牧兽医]