苦荞麦总黄酮对高脂血大鼠血脂和抗氧化作用的影响  

EFFECTS OF TOTAL FLAVONOIDS EXTRACT OF TARTARY BUCKWHEAT GERM ON SERUM LIPIDS AND ANTIOXIDATION IN HYPERCHOLESTEROLEMIC RATS

作　　者：王敏[1] 魏益民[1] 高锦明[2] 

机构地区：[1]西北农林科技大学食品科学与工程学院,杨凌712100 [2]西北农林科技大学生命学院,杨凌712100

出　　处：《营养学报》2006年第6期502-505,509,共5页Acta Nutrimenta Sinica

基　　金：科技部攻关计划重大项目(No.2003BA901A19);西安市2003年农业科技攻关计划项目(No.NG200317);西北农林科技大学2005年人才基金与校科研基金资助

摘　　要：目的:研究苦荞麸总黄酮对实验性高脂血症大鼠的降脂和抗氧化作用。方法:7w龄,60只Wistar大鼠,按体重随机分为6组;除正常对照组外,其余各组用高脂饲料制作动物高脂血症模型,其中苦荞麸总黄酮分为0.2、0.5、1.0g/kgbw剂量组;以绞股兰总苷片(0.032g/kgbw)为阳性对照组;正常组、模型组为10ml/kgbw蒸馏水,各组每天灌胃一次,连续6w。最终测定血脂、肝脂和抗氧化相关指标。结果:苦荞麸总黄酮各组与模型组比较:各剂量组均能有效地降低血清甘油三酯(TG)、总胆固醇(TC)和肝脏TC、TG值,其中小剂量组降血清TG、抗动脉硬化和抗血清脂质氧化效果显著,不仅使血清TG降低73.9%,TC降低36.4%,还能提高血清谷胱甘肽过氧化物酶(GSH-Px)活性和抗动脉粥样硬化指数(AAI)值,降低致动脉粥样硬化指数(AI)值和血清丙二醛(MDA)含量,且不引起肝脏指数(LI)变化。结论:苦荞麸总黄酮能显著降低大鼠血脂、肝脂水平,提高血清抗氧化酶活性,抑制血清脂质过氧化物产生。Objective： To study the effects of the total flavonoids of tartary buckwheat germ （TFTBG） on serum lipid and antioxidation in hypercholesterolemic rats. Method： According to body weight,60 Wistar rats were divided randomly into 6 groups： normal control group （NC）, high-fat control group （HFC）, Jiaogulan positive control group （JPC, Gynostemma pantaphyllum total glucoside tablet 0.032g/kg bw）,TFTBG 1.0,0.5 and TFTBG 0.2 g/kg bw （HD,MD,LD） group. Except NC group, all other groups were fed high-fat diet for hyperlipidemia model. NC group and HFC group were given distilled water 10 ml/kg bw. Water and TFTBG were given by gavage once a day for 6 w respectively. Serum TG, TC HDL-C, LDL-C, apoA1, apoB, SOD, GSH-Px and MDA were determined. Results： Compared with HFC group, serum TG ,TC were lowered significantly（P〈0.01,P〈0.01） in all TFTBG groups. Hepatic TC and TG were lowered significantly too. In LD group, serum GSH-Px and AAI were elevated, while serum MDA and AI were lowered. Conclusion： TFTBG can lower serum and hepatic lipid level and elevate the activity of antioxidative enzymes significantly. It has antioxidant and hypocholesterolemtic capacity in hypercholesterolemic rats.

关 键 词：苦荞麸总黄酮 血脂 肝脂 抗氧化酶 丙二醛 

分 类 号：R151[医药卫生—营养与食品卫生学] R589.2[医药卫生—公共卫生与预防医学]